# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5112525 | B | Inhibition of N-terminal His-6 tagged OTUB1 (40 to 271 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using Ub-Rho measured after 0.5 to 3 hrs by fluorescence based Plate reader method | Homo sapiens | 6 | ALA5108067 | cell-based format | Scientific Literature | |
2. | ALA5112555 | B | Binding affinity to OTUB1 in HEK293T cells at 2.5 uM preincubated for 30 mins followed by UV irradiation and measured after 20 mins by Western blot analysis | Homo sapiens | 1 | ALA5108067 | cell-based format | Scientific Literature | |
3. | ALA5112557 | B | Binding affinity to OTUB1 in HEK293T cells at 1 uM preincubated for 2 hrs followed by UV irradiation and measured after 20 mins by silver staining based gel electrophoresis assay | Homo sapiens | 1 | ALA5108067 | cell-based format | Scientific Literature | |
4. | ALA5112558 | B | Binding affinity to OTUB1 in HEK293T cells at 0.5 uM preincubated for 2 hrs followed by UV irradiation and measured after 20 mins by Western blot analysis | Homo sapiens | 1 | ALA5108067 | cell-based format | Scientific Literature |