| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5119580 | B | Inhibition of CDK8 (unknown origin) at 200 nM incubated in presence of ATP by ADP-Glo kinase assay relative to control | Homo sapiens | 94 | ALA5113525 | single protein format | Scientific Literature | |
| 2. | ALA5119581 | F | Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay | Homo sapiens | 24 | ALA5113525 | cell-based format | Scientific Literature | |
| 3. | ALA5119582 | F | Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay | Homo sapiens | 24 | ALA5113525 | cell-based format | Scientific Literature | |
| 4. | ALA5119583 | F | Antiproliferative activity against human SW480 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay | Homo sapiens | 24 | ALA5113525 | cell-based format | Scientific Literature | |
| 5. | ALA5119584 | F | Antiproliferative activity against mouse CT26 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay | Mus musculus | 24 | ALA5113525 | cell-based format | Scientific Literature | |
| 6. | ALA5119585 | T | Cytotoxicity against human GES1 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay | Homo sapiens | 24 | ALA5113525 | cell-based format | Scientific Literature | |
| 7. | ALA5119586 | B | Inhibition of CDK8 (unknown origin) incubated in presence of ATP by ADP-Glo kinase assay | Homo sapiens | 24 | ALA5113525 | single protein format | Scientific Literature | |
| 8. | ALA5119587 | B | Inhibition of CDK8 in human HCT-116 cells transfected with TOPFlash luciferase reporter plasmid assessed as reduction in TCF/LEF transcriptional activity | Homo sapiens | 13 | ALA5113525 | cell-based format | Scientific Literature | |
| 9. | ALA5119588 | B | Binding affinity to CDK8 in human HCT-116 cells assessed as thermal stability by measuring degradation temperature at 10 uM measured after 4 hrs by CETSA assay (Rvb = 49 degreeC) | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 10. | ALA5119589 | B | Inhibition of CDK8 in human HCT-116 cells assessed as reduction in STAT1 phosphorylation at S727 residue measured after 24 hrs by Western blot assay | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 11. | ALA5119590 | B | Binding affinity to CDK8 in human HCT-116 cells assessed as reduction in protease induced CDK8 degradation incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 12. | ALA5119591 | B | Inhibition of CDK8 in mouse BMDC cells assessed as reduction in IFNgamma-induced STAT1 phosphorylation at S727 residue pretreated with IFNgamma for 2 hrs followed by compound treatment for 24 hrs by Western blot assay | Mus musculus | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 13. | ALA5119592 | B | Inhibition of JAK in human HCT-116 cells assessed as reduction in STAT-1 phosphorylation at Tyr701 residue by Western blot assay | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 14. | ALA5119593 | B | Inhibition of JAK in mouse BMDC cells assessed as reduction in IFNgamma-induced STAT1 phosphorylation at Tyr701 residue pretreated with IFNgamma for 2 hrs followed by compound treatment for 24 hrs by Western blot assay | Mus musculus | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 15. | ALA5119594 | B | Inhibition of CDK8 in human HaCaT cells assessed as reduction in TGF-beta induced SMAD3 phosphorylation at thr179 residue pretreated with compound for 30 mins followed by TGF-beta treatment for 16 hrs by Western blot assay | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 16. | ALA5119595 | B | Inhibition of Wnt signaling pathway in human HCT-116 cells assessed as reduction in Wnt3alpha protein level upto 2 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 17. | ALA5119596 | B | Inhibition of TCF/LEF in human HCT-116 cells assessed as suppression of C-myc expression upto 2 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 18. | ALA5119597 | B | Inhibition of TCF/LEF in human HCT-116 cells assessed as suppression of cyclin D1 level expression upto 2 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 19. | ALA5119598 | B | Inhibition of beta-catenin in human HCT-116 cells assessed as accumulation in cytoplasm incubated for 24 hrs by DAPI staining based immunofluorescence assay | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature | |
| 20. | ALA5119599 | F | Induction of cell cycle arrest in human HCT-116 cells assessed as accumulation of diploid cells at G1 phase at 0.5 uM incubated for 24 hrs by PI staining based flow cytometric analysis (Rvb = 93.27 %) | Homo sapiens | 1 | ALA5113525 | cell-based format | Scientific Literature |
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