# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5109144 | Binding | Inhibition of RpoN-mediated GlnAp2 transcription in Escherichia coli XL-1 Blue expressing GFP assessed as decrease in GFP levels at 10 uM incubated for 4 hrs under minimal nitrogen media by fluorescence based assay relative to control | Escherichia coli | 33 | ALA5107971 | nucleic acid format | Scientific Literature | |
2. | ALA5109146 | Binding | Inhibition of sigma70-mediated lacUV5 transcription in Escherichia coli XL-1 Blue expressing GFP assessed as decrease in GFP levels at 10 uM incubated for 4 hrs under low nitrogen level in presence of IPTG by fluorescence based assay | Escherichia coli | 6 | ALA5107971 | nucleic acid format | Scientific Literature | |
3. | ALA5109147 | Binding | Binding affinity to FITC-labelled dsDNA (unknown origin) assessed as dissociation constant incubated for 10 mins by fluorescence polarization assay | Not specified | 8 | ALA5107971 | nucleic acid format | Scientific Literature | |
4. | ALA5109148 | Toxicity | Hemolytic activity in human RBC at 100 uM measured after 1 hrs relative to control | Homo sapiens | 8 | ALA5107971 | cell-based format | Scientific Literature | |
5. | ALA5109149 | Binding | Induction of inner membrane permeabilization in Escherichia coli ML35 at 10 uM incubated for 1 hrs by ONPG beta-galactosidase based assay | Escherichia coli | 7 | ALA5107971 | subcellular format | Scientific Literature | |
6. | ALA5109153 | Functional | Antibacterial activity against Pseudomonas aeruginosa assessed as inhibition of cell growth upto 32 ug/ml incubated overnight by microtiter broth dilution assay | Pseudomonas aeruginosa | 7 | ALA5107971 | organism-based format | Scientific Literature |