#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5099913	Binding	Agonist activity at NOD2 in HEK-Blue hNOD2 cells assessed as increase in NFkappaB activation incubated for 18 hrs by SEAP-based spectrophotometry analysis	Homo sapiens	3	ALA5096231	cell-based format	Scientific Literature
2.	ALA5099914	Binding	Agonist activity at TLR7 in HEK-Blue hTLR7 cells assessed as increase in NFkappaB activation incubated for 18 hrs by SEAP-based spectrophotometry analysis	Homo sapiens	3	ALA5096231	cell-based format	Scientific Literature
3.	ALA5099917	Binding	Agonist activity at NOD1 in HEK-Blue hNOD1 cells assessed as increase in NFkappaB activation at 10 uM incubated for 18 hrs by SEAP-based spectrophotometry analysis	Homo sapiens	2	ALA5096231	cell-based format	Scientific Literature
4.	ALA5099918	Binding	Activation of NF-kappaB transcriptional activity in mouse RAW-Blue cells expressing NOD2 and TLR7 at 1 uM incubated for 18 hrs by quanti-blue/SEAP-based spectrophotometry analysis	Mus musculus	2	ALA5096231	cell-based format	Scientific Literature
5.	ALA5099919	Binding	Activation of NF-kappaB transcriptional activity in mouse RAW-Blue cells expressing NOD2 and TLR7 at 1 uM preincubated with NOD2 antagonist, SG84 for 1 hr followed by compound addition and measured after 18 hrs by quanti-blue/SEAP-based spectrophotometry analysis	Mus musculus	2	ALA5096231	cell-based format	Scientific Literature
6.	ALA5099920	Binding	Activation of NF-kappaB transcriptional activity in mouse RAW-Blue cells expressing NOD2 and TLR7 at 1 uM preincubated with TLR7 antagonist, M5049 for 1 hr followed by compound addition and measured after 18 hrs by quanti-blue/SEAP-based spectrophotometry analysis	Mus musculus	2	ALA5096231	cell-based format	Scientific Literature
7.	ALA5099921	Binding	Activation of NF-kappaB transcriptional activity in mouse RAW-Blue cells expressing NOD2 and TLR7 at 1 uM preincubated with TLR7 antagonist, M5049 and NOD2 antagonist, SG84 for 1 hr followed by compound addition and measured after 18 hrs by quanti-blue/SEAP-based spectrophotometry analysis	Mus musculus	2	ALA5096231	cell-based format	Scientific Literature
8.	ALA5099922	Functional	Immunostimulatory activity in human PBMC cells assessed as increase in IL-1beta production at 1 uM incubated for 18 hrs by flow cytometry analysis	Homo sapiens	1	ALA5096231	cell-based format	Scientific Literature
9.	ALA5099923	Functional	Immunostimulatory activity in human PBMC cells assessed as increase in IL-6 production at 1 uM incubated for 18 hrs by flow cytometry analysis	Homo sapiens	1	ALA5096231	cell-based format	Scientific Literature
10.	ALA5099924	Functional	Immunostimulatory activity in human PBMC cells assessed as increase in IL-8 production at 1 uM incubated for 18 hrs by flow cytometry analysis	Homo sapiens	2	ALA5096231	cell-based format	Scientific Literature
11.	ALA5099925	Functional	Immunostimulatory activity in human PBMC cells assessed as increase in IL-10 production at 1 uM incubated for 18 hrs by flow cytometry analysis	Homo sapiens	1	ALA5096231	cell-based format	Scientific Literature
12.	ALA5099926	Functional	Immunostimulatory activity in human PBMC cells assessed as increase in TNF production at 1 uM incubated for 18 hrs by flow cytometry analysis	Homo sapiens	1	ALA5096231	cell-based format	Scientific Literature
13.	ALA5099927	Functional	Immunostimulatory activity in human PBMC cells assessed as increase in IL12p70 production at 1 uM incubated for 18 hrs by flow cytometry analysis	Homo sapiens	1	ALA5096231	cell-based format	Scientific Literature
14.	ALA5099928	Functional	Cytotoxicity against human K562 cells cocultured with human PBMC cells assessed as inhibition of cell proliferation at 0.01 to 1 uM incubated with PBMC cells for 18 hrs followed by co-incubation with K562 cells for 4 hrs by SYTOX blue staining based flow cytometric analysis	Homo sapiens	1	ALA5096231	cell-based format	Scientific Literature
15.	ALA5099929	Functional	Cytotoxicity against human MEC1 cells cocultured with human PBMC cells assessed as inhibition of cell proliferation at 0.01 to 1 uM incubated with PBMC cells for 18 hrs followed by co-incubation with MEC-1 cells for 4 hrs by SYTOX blue staining based flow cytometric analysis	Homo sapiens	1	ALA5096231	cell-based format	Scientific Literature
16.	ALA5099932	Functional	Induction of activation of human CD56+ CD3- NK cells co-cultured with human PBMC cells assessed as increase in CD69 expression at 1 uM incubated for 24 hrs by flow cytometry analysis (Rvb = 2.7%)	Homo sapiens	4	ALA5096231	cell-based format	Scientific Literature
17.	ALA5099933	Functional	Induction of activation of human CD56+ CD3- NK cells co-cultured with human PBMC cells assessed as increase in CD107a expression at 1 uM incubated for 24 hrs by flow cytometry analysis (Rvb = 1.5%)	Homo sapiens	4	ALA5096231	cell-based format	Scientific Literature
18.	ALA5099934	Functional	Induction of activation of human CD56+ CD3- NK cells co-cultured with human PBMC cells assessed as increase in IFN-gamma expression at 1 uM incubated for 24 hrs by flow cytometry analysis (Rvb = 0.04%)	Homo sapiens	4	ALA5096231	cell-based format	Scientific Literature
19.	ALA5099935	Functional	Induction of mouse ovalbumin specific CD4+ T cell activation assessed as increase in CD25 expression at 1 uM preincubated for 18 hrs with mouse BDMC cells in presence of ovalbumin followed by washout and further coincubated with CD4+ T cells and measured after 72 hrs by flow cytometry analysis (Rvb = 10.9%)	Mus musculus	4	ALA5096231	cell-based format	Scientific Literature
20.	ALA5099936	Functional	Induction of mouse ovalbumin specific CD8-positive T cell activation assessed as increase in CD25 expression at 1 uM preincubated for 18 hrs with mouse BDMC cells in presence of ovalbumin followed by washout and further coincubated with CD4+ T cells and measured after 72 hrs by flow cytometry analysis (Rvb = 12.6%)	Mus musculus	4	ALA5096231	cell-based format	Scientific Literature