#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5144467	B	Binding affinity to calf thymus DNA assessed as intercalation by measuring dissociation constant at 20 uM measured after 5 mins by UV-Vis spectrophotometric titration analysis	Bos taurus	7	ALA5143526	nucleic acid format	Scientific Literature
2.	ALA5144468	B	Binding affinity to calf thymus DNA assessed as ligand-induced thermal stabilization of DNA duplex by measuring change in melting temperature at 6.25 uM by thermal denaturation analysis	Bos taurus	7	ALA5143526	nucleic acid format	Scientific Literature
3.	ALA5144469	B	Displacement of ethidium bromide from calf thymus DNA incubated for 30 mins by fluorescence microplate reader assay	Bos taurus	7	ALA5143526	nucleic acid format	Scientific Literature
4.	ALA5144470	B	Binding affinity to calf thymus DNA assessed as ligand induced positive CD band at 50 uM by Circular dichroism spectroscopy analysis	Bos taurus	2	ALA5143526	nucleic acid format	Scientific Literature
5.	ALA5144471	B	Inhibition of human recombinant MGMT expressed in Escherichia coli STAR BL21 pRare cells assessed as cleavage of fluorescent labelled substrate by Bsp 119I at 12.5 uM using O6MeG-bearing substrate (S) incubated for 5 mins by coupled enzymatic analysis relative to control	Homo sapiens	14	ALA5143526	cell-based format	Scientific Literature
6.	ALA5144472	B	Irreversible inhibition of human recombinant MGMT expressed in Escherichia coli STAR BL21 pRare cells assessed as cleavage of fluorescent labelled substrate by Bsp 119I at 12.5 uM using O6MeG-bearing substrate (S) preincubated for 30 mins followed by substrate addition and measured after 5 mins by coupled enzymatic analysis relative to control	Homo sapiens	8	ALA5143526	cell-based format	Scientific Literature
7.	ALA5144474	B	Competitive inhibition of human recombinant MGMT expressed in Escherichia coli STAR BL21 pRare cells assessed as cleavage of fluorescent labelled substrate by Bsp 119I at 12.5 uM using O6MeG-bearing substrate (S) incubated for 10 mins in presence of non-substrate CT-DNA followed by substrate addition and measured after 5 mins by coupled enzymatic analysis	Homo sapiens	7	ALA5143526	cell-based format	Scientific Literature
8.	ALA5144475	F	Antiproliferative activity against human T98G cells assessed as cell growth inhibition incubated for 96 hrs by CellTiter-Glo luminescent cell viability assay	Homo sapiens	14	ALA5143526	cell-based format	Scientific Literature
9.	ALA5144482	A	Drug localization in cytosolic region of human T98G cells at 1.1 uM measured after 96 hrs by confocal microscope based live cell imaging analysis	Homo sapiens	1	ALA5143526	subcellular format	Scientific Literature
10.	ALA5144483	A	Drug localization in nucleus of human T98G cells at 1.1 uM measured after 96 hrs by confocal microscope based live cell imaging analysis	Homo sapiens	1	ALA5143526	subcellular format	Scientific Literature
11.	ALA5144484	A	Drug localization in perinuclear region of nuclear membrane of human T98G cells assessed as appearance of z-stacked series at 1.1 uM measured after 96 hrs by confocal microscope based live cell imaging analysis	Homo sapiens	1	ALA5143526	cell membrane format	Scientific Literature
12.	ALA5144485	B	Inactivation of MGMT in human T98G cells assessed as reduction in protein content in cellular extract at 2 uM using O6MeG-bearing substrate (S) incubated for 2 hrs by PAGE analysis relative to control	Homo sapiens	2	ALA5143526	cell-based format	Scientific Literature
13.	ALA5144488	F	Induction of DNA damage in human T98G cells assessed as increase in gammaH2AX foci number and size at 1.1 uM pretreated for 2 hrs followed by TMZ addition and measured after 94 hrs by DAPI staining based immunofluorescence assay	Homo sapiens	1	ALA5143526	cell-based format	Scientific Literature
14.	ALA5144489	F	Induction of DNA damage in human T98G cells assessed as increase in gammaH2AX foci number and size at 1.1 uM measured after 96 hrs by DAPI staining based immunofluorescence assay	Homo sapiens	1	ALA5143526	cell-based format	Scientific Literature
15.	ALA5144494	F	Induction of apoptosis in human T98G cells assessed as increase in early apoptotic cells at 1.1 uM measured after 48 hrs by Annexin V-FITC/PI staining based flow cytometry analysis	Homo sapiens	1	ALA5143526	cell-based format	Scientific Literature
16.	ALA5144495	F	Induction of apoptosis in human T98G cells assessed as increase in early apoptotic cells at 1.1 uM measured after 96 hrs by Annexin V-FITC/PI staining based flow cytometry analysis relative to control	Homo sapiens	1	ALA5143526	cell-based format	Scientific Literature
17.	ALA5144496	F	Induction of apoptosis in human T98G cells assessed as increase in early apoptotic cells at 1.1 uM pretreated for 2 hrs followed by TMZ addition and measured after 96 hrs by Annexin V-FITC/PI staining based flow cytometry analysis relative to control	Homo sapiens	1	ALA5143526	cell-based format	Scientific Literature
18.	ALA5144497	F	Induction of apoptosis in human T98G cells assessed as fold increase in caspase activation at 1.1 uM measured after 96 hrs by Caspase3/7 detection reagent based assay relative to control	Homo sapiens	1	ALA5143526	cell-based format	Scientific Literature
19.	ALA5144498	F	Induction of apoptosis in human T98G cells assessed as increase in caspase activation at 1.1 uM pretreated for 2 hrs followed by TMZ addition and measured after 94 hrs by Caspase3/7 detection reagent based assay	Homo sapiens	1	ALA5143526	cell-based format	Scientific Literature
20.	ALA5144499	F	Synergistic antiproliferative activity against human T98G cells assessed as reduction in cell viability at GI50 pretreated for 2 hrs followed by TMZ addition at 94 uM and measured after 94 hrs by CellTiter-Glo luminescent cell viability assay	Homo sapiens	3	ALA5143526	cell-based format	Scientific Literature