# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5154067 | B | Inhibition of recombinant full length C-terminal His/Flag-tagged human HDAC1 (1 to 482 residues) using Z-Lys(Ac)-AMC as substrate preincubated for 90 mins followed by trypsin addition and measured after 30 mins by fluorescence based microplate reader analysis | Homo sapiens | 11 | ALA5150069 | single protein format | Scientific Literature | |
2. | ALA5154068 | B | Inhibition of recombinant full length N-terminal GST-tagged human HDAC6 (1 to 1215 residues) using Z-Lys(Ac)-AMC as substrate preincubated for 90 mins followed by trypsin addition and measured after 30 mins by fluorescence based microplate reader analysis | Homo sapiens | 11 | ALA5150069 | single protein format | Scientific Literature | |
3. | ALA5154069 | B | Selectivity index, ratio of IC50 for human HDAC1 to IC50 for human HDAC6 | Homo sapiens | 11 | ALA5150069 | assay format | Scientific Literature | |
4. | ALA5154070 | B | Induction of HDAC1 degradation in human HL-60 cells at 0.1 to 10 uM after 6 hrs by Western blot analysis | Homo sapiens | 3 | ALA5150069 | cell-based format | Scientific Literature | |
5. | ALA5154071 | B | Induction of HDAC6 degradation in human HL-60 cells at 0.1 to 10 uM after 6 hrs by Western blot analysis | Homo sapiens | 3 | ALA5150069 | cell-based format | Scientific Literature | |
6. | ALA5154072 | B | Induction of HDAC6 degradation in human HL-60 cells assessed as hyperacetylation of alpha-tubulin at 0.1 to 10 uM after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
7. | ALA5154073 | B | Induction of HDAC4 degradation in human HL-60 cells at 0.1 to 10 uM after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
8. | ALA5154074 | B | Induction of HDAC1 degradation in human HL-60 cells assessed as hyperacetylation of histone H3 at 0.1 to 10 uM after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
9. | ALA5154075 | B | Induction of Hitbit-fused HDAC6 degradation in human K562 cells at 1 uM after 5.5 to 7.5 hrs by NanoGlo HiBit lytic assay | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
10. | ALA5154076 | B | Induction of HDAC6 degradation in human U-266 cells at 100 to 1000 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
11. | ALA5154077 | B | Induction of HDAC6 degradation in human U-266 cells assessed as hyperacetylation of alpha-tubulin at 100 to 1000 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
12. | ALA5154078 | B | Induction of HDAC6 degradation in human HL-60 cells after 6 hrs by Western immunoassay | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
13. | ALA5154079 | B | Induction of HDAC6 degradation in human HL-60 cells assessed as maximum degradation after 6 hrs by Western immunoassay | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
14. | ALA5154080 | B | Induction of HDAC6 degradation in human HL-60 cells assessed as hyperacetylation of alpha-tubulin after 6 hrs by Western immunoassay | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
15. | ALA5154081 | B | Induction of HDAC6 degradation in human HL-60 cells pretreated with MG132 for 1 hr followed by compound addition and measured after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
16. | ALA5154082 | B | Induction of HDAC6 degradation in human HL-60 cells pretreated with vorinostat for 1 hr followed by compound addition and measured after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
17. | ALA5154083 | B | Induction of HDAC6 degradation in human HL-60 cells pretreated with nextura-stat for 1 hr followed by compound addition and measured after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
18. | ALA5154084 | B | Induction of HDAC6 degradation in human HL-60 cells pretreated with pomalidomide for 1 hr followed by compound addition and measured after 6 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150069 | cell-based format | Scientific Literature | |
19. | ALA5154089 | F | Cytotoxicity against human 697 cells assessed as reduction in cell viability at 0.5 to 50 uM incubated for 72 hrs by ATP-Glo based assay | Homo sapiens | 1 | ALA5150069 | cell-based format | Scientific Literature | |
20. | ALA5154090 | F | Cytotoxicity against human REH cells assessed as reduction in cell viability at 0.5 to 50 uM incubated for 72 hrs by ATP based Celltiter-glo luminescent assay | Homo sapiens | 1 | ALA5150069 | cell-based format | Scientific Literature |