| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5153970 | F | Inhibition of Hedgehog-signalling dependent purmorphamine induced osteoblast differentiation in mouse C3H 10T1/2 cells assessed as residual activity by measuring ALP expression at 10 uM incubated for 96 hrs by CDP-Star luminogenic substrate based assay relative to control | Mus musculus | 25 | cell-based format | Scientific Literature | ||
| 2. | ALA5153971 | F | Inhibition of Hedgehog-signalling dependent purmorphamine induced osteoblast differentiation in mouse C3H 10T1/2 cells assessed as reduction in ALP expression incubated for 96 hrs by CDP-Star luminogenic substrate based assay | Mus musculus | 10 | cell-based format | Scientific Literature | ||
| 3. | ALA5153972 | F | Inhibition of Hedgehog-signalling dependent purmorphamine induced osteoblast differentiation in mouse C3H 10T1/2 cells assessed as inhibition of ALPL gene expression incubated for 96 hrs by RT-qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA5153973 | F | Inhibition of osteoblast differentiation in mouse C3H 10T1/2 cells assessed as suppression of bone mineralization at 0.0001 to 1 uM incubated for 21 days in Shh-conditioned medium with media replacement for every 3 days by Alizarin Red S staining-based microscopic analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA5153974 | T | Cytotoxicity against mouse C3H 10T1/2 cells assessed as reduction in cell viability at 10 uM measured every 2 hrs for 72 hrs by IncuCyte zoom live-cell imaging analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA5153975 | B | Inhibition of hedgehog signalling in purmorphamine treated mouse C3H 10T1/2 cells assessed as modulation of Ptch1 gene expression at 0.1 to 1 uM incubated for 96 hrs by RT-qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA5153976 | B | Inhibition of hedgehog signalling in purmorphamine treated mouse C3H 10T1/2 cells assessed as modulation of Gli1 gene expression at 0.1 to 1 uM incubated for 96 hrs by RT-qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA5153977 | B | Inhibition of hedgehog signalling in purmorphamine treated mouse C3H 10T1/2 cells assessed as modulation of Gli2 gene expression at 0.1 to 1 uM incubated for 96 hrs by RT-qPCR analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA5153978 | F | Inhibition of Wnt3A-induced osteoblast differentiation in mouse C3H 10T1/2 cells assessed as reduction in alkaline phosphatase activity incubated for 12 days by CDP-Star luminogenic substrate based assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA5153979 | B | Inhibition of Wnt-3a induced Wnt signalling in HEK293T cells assessed as modulation of TCF/LEF dependent reporter activity at 0.01 to 100 uM incubated for 24 hrs by Renilla/Firefly based dual-luciferase reporter assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA5153980 | F | Inhibition of BMP4-induced osteoblast differentiation in mouse C2C12 cells at 0.00001 to 1 uM incubated for 72 hrs by CDP-Star luminogenic substrate based assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA5153981 | F | Downregulation of ALPL gene expression in purmorphamine treated mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA5153982 | F | Downregulation of purmorphamine-induced Gli1 gene expression in mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA5153983 | F | Downregulation of purmorphamine-induced Ptch1 gene expression in mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5153984 | F | Upregulation of Cyp1B1 gene expression in purmorphamine treated mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5153985 | F | Upregulation of Aldh3a1 gene expression in purmorphamine treated mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA5153986 | F | Upregulation of Aldh7 gene expression in purmorphamine treated mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5153987 | F | Upregulation of Nqo1 gene expression in purmorphamine treated mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA5153988 | F | Upregulation of Xdh gene expression in purmorphamine treated mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA5153989 | F | Upregulation of Tiparp gene expression in purmorphamine treated mouse C3H 10T1/2 cells at 1 to 100 nM measured upto 7 days by global transcriptome analysis | Mus musculus | 1 | cell-based format | Scientific Literature |
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