# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5100168 | B | Inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
2. | ALA5100169 | B | Inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
3. | ALA5100170 | B | Selectivity index, ratio of IC50 for inhibition of recombinant human C-terminal FLAG/His-tagged HDAC1 (1 to 482 residues) expressed in Sf9 insect cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
4. | ALA5100171 | B | Inhibition of recombinant human C-terminal FLAG-tagged HDAC2 (1 to 488 residues) expressed in Sf9 insect cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
5. | ALA5100172 | B | Inhibition of C-terminal His-tagged recombinant human HDAC3 (1 to 428 residues)/N-terminal GST-tagged recombinant human NCoR2 (395 to 489 residues) co-expressed in Sf9 cells using Z-Lys(Ac)-AMC as fluorogenic substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
6. | ALA5100173 | B | Inhibition of recombinant human N-terminal GST-tagged/C-terminal His-tagged HDAC4 (627 to 1084 residues) expressed in baculovirus infected Sf9 insect cells using Boc-Lys (trifluoroacetyl)-AMC as substrate incubated for 90 mins by microplate reader analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
7. | ALA5100174 | B | Selectivity index, ratio of IC50 for inhibition of recombinant human C-terminal FLAG-tagged HDAC2 (1 to 488 residues) expressed in Sf9 insect cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
8. | ALA5100175 | B | Selectivity index, ratio of IC50 for inhibition of C-terminal His-tagged recombinant human HDAC3 (1 to 428 residues)/N-terminal GST-tagged recombinant human NCoR2 (395 to 489 residues) co-expressed in Sf9 cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
9. | ALA5100176 | B | Selectivity index, ratio of IC50 for inhibition fo recombinant human N-terminal GST-tagged/C-terminal His-tagged HDAC4 (627 to 1084 residues) expressed in baculovirus infected Sf9 insect cells to IC50 for inhibition of recombinant human N-terminal GST-tagged HDAC6 (1 to 1215 residues) expressed in Sf9 insect cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA5100177 | F | Cytotoxicity against human HAL-01 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
11. | ALA5100178 | F | Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
12. | ALA5100179 | F | Cytotoxicity against human Jurkat cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
13. | ALA5100180 | F | Induction of apoptosis in human HL-60 cells assessed as increase in total apoptotic cells at 1 to 5 uM incubated for 48 hrs by annexin V and propidium iodide staining based flow cytometry analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
14. | ALA5100181 | B | Inhibition of HDAC in human HL-60 cells assessed as increase in alpha tubulin acetylation incubated for 24 hrs by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
15. | ALA5100182 | B | Inhibition of HDAC in human HL-60 cells assessed as increase in Ac-H3 levels incubated for 24 hrs by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
16. | ALA5100185 | F | Synergistic cytotoxicity against human HL-60 cells assessed as increase in synergy score at 0.1 to 2 uM incubated for 72 hrs in presence of DNA methyltransferase inhibitor, decitabine by CellTiter-Glo luminescent assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
17. | ALA5100186 | F | Synergistic cytotoxicity against human MOLM-13 cells assessed as increase in synergy score at 0.1 to 2 uM incubated for 72 hrs in presence of NA methyltransferase inhibitor, decitabine by CellTiter-Glo luminescent assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
18. | ALA5100187 | F | Synergistic cytotoxicity against human HAL-01 cells assessed as increase in synergy score at 0.1 to 2 uM incubated for 72 hrs in presence of NA methyltransferase inhibitor, decitabine by CellTiter-Glo luminescent assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
19. | ALA5100188 | F | Synergistic cytotoxicity against human Jurkat cells assessed as increase in synergy score at 0.1 to 2 uM incubated for 72 hrs in presence of NA methyltransferase inhibitor, decitabine by CellTiter-Glo luminescent assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
20. | ALA5100191 | B | Inhibition of HDAC in human HL-60 cells assessed as increase in Ac-H3 levels at 0.15 uM incubated for 24 hrs in presence of DNA methyltransferase inhibitor, decitabine by immunoblot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |