# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5124302 | F | Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 5 uM preincubated with compound for 2 hrs followed by LPS stimulation for 24 hrs by Griess reagent based assay relative to control | Mus musculus | 49 | cell-based format | Scientific Literature | ||
2. | ALA5124303 | F | Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated with compound for 2 hrs followed by LPS stimulation for 24 hrs by Griess reagent based assay | Mus musculus | 13 | cell-based format | Scientific Literature | ||
3. | ALA5124304 | T | Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability at 5 uM incubated for 24 hrs by alamar blue reagent based fluorescence assay | Mus musculus | 49 | cell-based format | Scientific Literature | ||
4. | ALA5124305 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production preincubated for 2 hrs followed by LPS-stimulation for 24 hrs by ELISA | Mus musculus | 2 | cell-based format | Scientific Literature | ||
5. | ALA5124306 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-1beta production preincubated for 2 hrs followed by LPS-stimulation for 24 hrs by ELISA | Mus musculus | 2 | cell-based format | Scientific Literature | ||
6. | ALA5124307 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced IL-6 mRNA expression at 0.2 to 5 uM preincubated for 2 hrs followed by LPS-stimulation for 24 hrs by qRT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
7. | ALA5124308 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced IL-1beta mRNA expression at 0.2 to 5 uM preincubated for 2 hrs followed by LPS-stimulation for 24 hrs by qRT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
8. | ALA5124309 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced iNOS mRNA expression preincubated for 2 hrs followed by LPS stimulation for 24 hrs by qRT-PCR analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
9. | ALA5124310 | F | Antiinflammatory activity in mouse peritoneal macrophages assessed as reduction in LPS-induced NO production at 0.2 to 5 uM preincubated for 2 hrs followed by LPS-stimulation for 22 hrs by Griess reagent based assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
10. | ALA5124311 | F | Antiinflammatory activity in human THP-1 monocytes assessed as reduction in LPS/Interferon-gamma NO production at 0.2 to 5 uM pretreated with PMA for 24 hrs followed by compound addition 2 hrs and stimulated with LPS/Interferon-gamma for 22 hrs by Griess reagent based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
11. | ALA5124312 | B | Activation of NF-kappaB signalling pathway in mouse RAW264.7 cells assessed as suppression of LPS-induced IkappaBalpha phosphorylation pretreated with compound for 2 hrs followed by LPS-stimulation for 30 mins by Western blot analysis | Mus musculus | 3 | cell-based format | Scientific Literature | ||
12. | ALA5124313 | B | Activation of NF-kappaB signalling pathway in mouse RAW264.7 cells assessed as suppression of LPS-induced IkappaBalpha degradation pretreated with compound for 2 hrs followed by LPS-stimulation for 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
13. | ALA5124314 | B | Activation of NF-kappaB signalling pathway in mouse RAW264.7 cells assessed as decrease in LPS-induced AKT phosphorylation at 0.2 to 5 uM pretreated with compound for 2 hrs followed by LPS-stimulation for 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
14. | ALA5124315 | B | Inhibition of LPS-induced p38 MAPK phosphorylation in mouse RAW264.7 cells at 0.2 to 5 uM pretreated with compound for 2 hrs followed by LPS-stimulation for 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
15. | ALA5124316 | B | Inhibition of LPS-induced ERK1/2 MAPK phosphorylation in mouse RAW264.7 cells at 0.2 to 5 uM pretreated with compound for 2 hrs followed by LPS-stimulation for 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
16. | ALA5124317 | B | Inhibition of LPS-induced JNK MAPK phosphorylation in mouse RAW264.7 cells at 0.2 to 5 uM pretreated with compound for 2 hrs followed by LPS-stimulation and measured after 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
17. | ALA5124318 | B | Inhibition of LPS-induced STAT3 phosphorylation in mouse RAW264.7 cells pretreated with compound for 2 hrs followed by LPS-stimulation and measured after 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
18. | ALA5124319 | B | Inhibition of LPS-induced STAT1 phosphorylation in mouse RAW264.7 cells pretreated with compound for 2 hrs followed by LPS-stimulation and measured after 30 mins by immunoblot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
19. | ALA5124320 | F | Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as enhancement of JAK2 phosphorylation pretreated with compound for 2 hrs followed by LPS-stimulation and measured after 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature | ||
20. | ALA5124321 | F | Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as enhancement of STAT3 phosphorylation pretreated with compound for 2 hrs followed by LPS-stimulation and measured after 30 mins by Western blot analysis | Mus musculus | 2 | cell-based format | Scientific Literature |