# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5136282 | Binding | PROTAC activity at CRBN/EGFR Del19 mutant in human HCC827 cells assessed as EGFR degradation treated after 8 hrs under serum starvation condition at 10 nM measured after 16 hrs by Western blotting analysis | Homo sapiens | 2 | ALA5131542 | cell-based format | Scientific Literature | |
2. | ALA5136283 | Functional | Antiproliferative activity against human HCC827 cells assessed as cell growth inhibition incubated for 3 days by CCK-8 assay | Homo sapiens | 16 | ALA5131542 | cell-based format | Scientific Literature | |
3. | ALA5136284 | Binding | Binding affinity to human wild type partial length EGFR (R669 to V1011 residues) expressed in bacterial expression system assessed as immobilized ligand binding by competitive binding assay | Homo sapiens | 4 | ALA5131542 | assay format | Scientific Literature | |
4. | ALA5136285 | Binding | Binding affinity to human partial length EGFR (R669 to V1011 residues) L858R mutant expressed in bacterial expression system assessed as immobilized ligand binding by competitive binding assay | Homo sapiens | 4 | ALA5131542 | assay format | Scientific Literature | |
5. | ALA5136287 | Binding | PROTAC activity at CRBN/EGFR Del19 mutant in human HCC827 cells assessed as EGFR degradation treated after 8 hrs under serum starvation condition measured after 16 hrs by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
6. | ALA5136289 | Binding | PROTAC activity at CRBN/EGFR Del19 mutant in human HCC827 cells assessed as inhibition of EGFR phosphorylation treated after 8 hrs under serum starvation condition measured after 16 hrs by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
7. | ALA5136290 | Binding | PROTAC activity at CRBN/EGFR in human HCC827 cells assessed as EGFR degradation at 100 nM treated after 8 hrs under serum starvation condition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
8. | ALA5136292 | Binding | PROTAC activity at CRBN/EGFR in human HCC827 cells assessed as EGFR degradation at 100 nM treated after 4 hrs under serum starvation condition and preincubated with NEDD8 inhibitor MLN4924 for 2 hrs measured after 8 hrs of compound addition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
9. | ALA5136294 | Binding | PROTAC activity at CRBN/EGFR Del19 mutant in human HCC827 cells assessed as EGFR degradation at 100 nM treated after 4 hrs under serum starvation condition and preincubated with BoA1 inhibitor for 2 hrs measured after 8 hrs of compound addition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
10. | ALA5136296 | Binding | PROTAC activity at wild type EGFR/CRBN overexpressed in human OVCAR-8 cells assessed as EGFR degradation upto 10000 nM treated after 8 hrs under serum starvation condition measured after 16 hrs of compound addition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
11. | ALA5136304 | Binding | PROTAC activity at CRBN/wild type EGFR overexpressed in human OVCAR-8 cells assessed as EGFR degradation upto 10000 nM treated after 8 hrs under serum starvation condition and preincubated with 5 uM pictilisib measured for 2 hrs after 16 hrs of compound addition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
12. | ALA5136306 | Binding | PROTAC activity at CRBN/wild type EGFR overexpressed in human NCI-H1299 cells assessed as EGFR degradation upto 10000 nM treated after 8 hrs under serum starvation condition and preincubated with 5 uM pictilisib measured for 2 hrs after 16 hrs of compound addition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
13. | ALA5136319 | Functional | Antiproliferative activity against human OVCAR-8 cells expressing wild type EGFR assessed as cell growth inhibition at 1 uM in presence of pictilisib | Homo sapiens | 4 | ALA5131542 | cell-based format | Scientific Literature | |
14. | ALA5136320 | Functional | Antiproliferative activity against human NCI-H1299 cells expressing wild type EGFR assessed as cell growth inhibition at 1 uM in presence of pictilisib | Homo sapiens | 4 | ALA5131542 | cell-based format | Scientific Literature | |
15. | ALA5136324 | Binding | PROTAC activity at CRBN/EGFR in human HCC827 cells assessed as EGFR degradation at 100 nM treated after 4 hrs under serum starvation condition and preincubated with gefitinib for 2 hrs measured after 8 hrs of compound addition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature |