| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5149637 | B | Inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by activation with ERK2 and substrate addition measured after 2 hrs by microfluidic chip based electrophoresis | Homo sapiens | 27 | ALA5143742 | cell-based format | Scientific Literature | |
| 2. | ALA5149638 | B | Inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate measured after 2 hrs in presence of ERK2 by microfluidic chip based electrophoresis | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 3. | ALA5149639 | B | Inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 3 hrs followed by activation with ERK2 and substrate addition measured after 2 hrs by microfluidic chip based electrophoresis | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 4. | ALA5149640 | B | Inhibition of MSK2 (unknown origin) FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by activation with ERK2 and substrate addition measured after 2 hrs by microfluidic chip based electrophoresis | Homo sapiens | 5 | ALA5143742 | cell-based format | Scientific Literature | |
| 5. | ALA5149641 | B | Inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by activation with ERK2 and substrate addition measured after 2 hrs in presence of 1 mM ATP by microfluidic chip based electrophoresis | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 6. | ALA5149642 | B | Inhibition of N-terminal His6-tagged recombinant human MSK1 (2 to end residues) expressed in baculovirus infected Sf21 insect cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by substrate addition and measured after 2 hrs by caliper method | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 7. | ALA5149643 | B | Inhibition of N-terminal His-TEV-Avi-tagged MSK1 N-terminal kinase domain (2 to 380 residues) (unknown origin) expressed in baculovirus infected Sf9 insect cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by substrate addition and measured after 70 mins by ADP-Glo kinase assay | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 8. | ALA5149644 | B | Inhibition of N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by substrate addition and measured after 70 mins in presence of 5 uM by ADP-Glo kinase assay | Homo sapiens | 5 | ALA5143742 | assay format | Scientific Literature | |
| 9. | ALA5149645 | B | Inhibition of N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by substrate addition and measured after 70 mins in presence of 250 uM by ADP-Glo kinase assay | Homo sapiens | 5 | ALA5143742 | assay format | Scientific Literature | |
| 10. | ALA5149646 | B | Selectivity ratio of IC50 for inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by activation with ERK2 and substrate addition measured after 2 hrs by microfluidic chip based electrophoresis to IC50 for Inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 3 hrs followed by activation with ERK2 and substrate addition measured after 2 hrs by microfluidic chip based electrophoresis | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 11. | ALA5149647 | B | Selectivity ratio of IC50 for inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate measured after 2 hrs in presence of ERK2 by microfluidic chip based electrophoresis to IC50 for Inhibition of full-length N-terminal His-TEV-tagged inactive form of MSK1 (2 to 802 residues) (unknown origin) expressed in HEK293 cells using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 3 hrs followed by activation with ERK2 and substrate addition measured after 2 hrs by microfluidic chip based electrophoresis | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 12. | ALA5149648 | B | Inhibition of N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate measured after 70 mins by ADP-Glo kinase assay | Homo sapiens | 1 | ALA5143742 | cell-based format | Scientific Literature | |
| 13. | ALA5149649 | B | Inhibition of N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) using FAM-PSKPAATRKRRWSAPESR-NH2 as substrate preincubated for 1 hr followed by substrate addition and measured after 70 mins by ADP-Glo kinase assay | Homo sapiens | 2 | ALA5143742 | cell-based format | Scientific Literature | |
| 14. | ALA5149654 | B | Binding affinity to N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) at 1:1 ratio of enzyme to compound measured after 1 min by native mass spectrometry relative to control | Homo sapiens | 5 | ALA5143742 | assay format | Scientific Literature | |
| 15. | ALA5149655 | B | Binding affinity to N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) at 1:1 ratio of enzyme to compound measured after 1 hr by native mass spectrometry relative to control | Homo sapiens | 5 | ALA5143742 | assay format | Scientific Literature | |
| 16. | ALA5149660 | B | Covalent binding affinity to N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) at 1:1 ratio of enzyme to compound measured after 1 min by LC-MS analysis relative to control | Homo sapiens | 5 | ALA5143742 | assay format | Scientific Literature | |
| 17. | ALA5149661 | B | Covalent binding affinity to N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) at 1:1 ratio of enzyme to compound measured after 1 hr by LC-MS analysis relative to control | Homo sapiens | 5 | ALA5143742 | assay format | Scientific Literature | |
| 18. | ALA5149662 | B | Covalent binding affinity to N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) at 1:1 ratio of enzyme to compound measured after 24 hrs by LC-MS analysis relative to control | Homo sapiens | 5 | ALA5143742 | assay format | Scientific Literature | |
| 19. | ALA5149663 | B | Covalent binding affinity to N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) at 1:2 ratio of enzyme to compound measured after 24 hrs by LC-MS analysis relative to control | Homo sapiens | 2 | ALA5143742 | assay format | Scientific Literature | |
| 20. | ALA5149664 | B | Covalent binding affinity to N-terminal His-Smt-tagged MSK1 C-terminal kinase domain (414 to 738 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) at 1:3 ratio of enzyme to compound measured after 24 hrs by LC-MS analysis relative to control | Homo sapiens | 2 | ALA5143742 | assay format | Scientific Literature |
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