| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5166836 | B | Binding affinity to MAGEA4 (unknown origin) expressed in Escherichia coli BL21-(DE3) by isothermal titration calorimetry | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 2. | ALA5166837 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain (unknown origin) expressed in Escherichia coli BL21-(DE3) by isothermal titration calorimetry | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 3. | ALA5166838 | B | Inhibition of biotinylated Eu-W1024 streptavidin-labeled MAGEA4 (unknown origin) expressed in Escherichia coli BL21-(DE3)/GST-tagged RAD18 (unknown origin) (267 to 402 residues) interaction using Ulight-anti-GST antibody incubated for 30 to 60 mins by LANCE TR-FRET assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 4. | ALA5166839 | B | Inhibition of Eu-W1024 streptavidin-labeled MAGEA4 (unknown origin) expressed in Escherichia coli BL21-(DE3) incubated for 30 to 60 mins by LANCE TR-FRET assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
| 5. | ALA5166840 | B | Inhibition of MAGEA4 in human NCI-H1299 cell lysate interaction with HA-tagged RAD18 (unknown origin) at 20 nM incubated for overnight by co-immunoprecipitation pull down assay | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 6. | ALA5166841 | B | Inhibition of MAGEA4 in human NCI-H1299 cell lysate interaction with HA-tagged RAD18 (unknown origin) at 1 uM incubated for overnight by co-immunoprecipitation pull down assay | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 7. | ALA5166842 | B | Inhibition of HA-tagged RAD18 interaction with RAD6A in human NCI-H1299 cell lysate at 1 to 1000 nM incubated for overnight by co-immunoprecipitation based western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
| 8. | ALA5166843 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as protection in trypsin-induced MAGEA4 protein degradation incubated for 1 hr by proteolysis based SDS-PAGE analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 9. | ALA5166844 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain M161A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA5166845 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain I205A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA5166846 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain E225A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA5166847 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain V228A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA5166848 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain M229A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA5166849 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain V291A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 15. | ALA5166850 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain V294A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 16. | ALA5166851 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain R297A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA5166852 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain R299A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 18. | ALA5166853 | B | Binding affinity to recombinant N-terminal His-tagged MAGEA4-MH domain D115R mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) assessed as increase in thermal stability by SYPRO orange dye based differential scanning fluorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 19. | ALA5166855 | B | Inhibition of Eu-W1024 streptavidin-labeled 6 xHis-tagged MAGEA4-MH domain I205A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) interaction with Ulight-labeled GST-tagged RAD18 (unknown origin) (267 to 402 residues) at 1 uM by LANCE TR-FRET assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 20. | ALA5166856 | B | Inhibition of Eu-W1024 streptavidin-labeled 6 xHis-tagged MAGEA4-MH domain M161A mutant (unknown origin) expressed in Escherichia coli BL21-(DE3) interaction with Ulight-labeled GST-tagged RAD18 (unknown origin) (267 to 402 residues) at 1 uM by LANCE TR-FRET assay | Homo sapiens | 1 | assay format | Scientific Literature |
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