# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5141582 | Binding | Activation of basal state recombinant PKG1alpha (unknown origin) using Glass-tide peptide substrate incubated for 30 mins followed by ATP addition and measured after 2 hr by ADP-GLO Max Assay | Homo sapiens | 42 | ALA5137073 | single protein format | Scientific Literature | |
2. | ALA5141583 | Binding | Activation of cGMP induced partially activated state recombinant PKG1alpha (unknown origin) using Glass-tide peptide substrate incubated for 30 mins followed by ATP addition and measured after 2 hr in presence of cGMP by ADP-GLO Max Assay | Homo sapiens | 42 | ALA5137073 | single protein format | Scientific Literature | |
3. | ALA5141584 | Physicochemical | Solubility of compound at pH 7 | 5 | ALA5137073 | small-molecule physicochemical format | Scientific Literature | ||
4. | ALA5141585 | ADME | Apparent permeability in dog MDCK-II cells | Canis lupus familiaris | 5 | ALA5137073 | cell-based format | Scientific Literature | |
5. | ALA5141586 | Binding | Activation of PKG1alpha in human HEK293 cells stably expressing VASP assessed as VSAP phosphorylation at Ser 239 incubated for 45 mins by HTRF assay | Homo sapiens | 5 | ALA5137073 | cell-based format | Scientific Literature | |
6. | ALA5141587 | Physicochemical | Solubility of compound at pH 2 | 3 | ALA5137073 | small-molecule physicochemical format | Scientific Literature | ||
7. | ALA5141588 | Physicochemical | Solubility of compound in FaSSIF | 3 | ALA5137073 | small-molecule physicochemical format | Scientific Literature | ||
8. | ALA5141593 | ADME | Clearance in Wistar Han male rat plasma at 0.05 mg/kg, iv and measured upto 8 hrs post dose by LC-MS/MS analysis | Rattus norvegicus | 2 | ALA5137073 | organism-based format | Scientific Literature | |
9. | ALA5141594 | ADME | Volume of distribution in Wistar Han male rat plasma at 0.05 mg/kg, iv and measured upto 8 hrs post dose by LC-MS/MS analysis | Rattus norvegicus | 2 | ALA5137073 | organism-based format | Scientific Literature | |
10. | ALA5141595 | ADME | MRT in Wistar Han male rat plasma at 0.05 mg/kg, iv and measured upto 8 hrs post dose by LC-MS/MS analysis | Rattus norvegicus | 2 | ALA5137073 | organism-based format | Scientific Literature | |
11. | ALA5141596 | ADME | Protein binding in Wistar Han male rat plasma at 0.05 mg/kg, iv and measured upto 8 hrs post dose by LC-MS/MS analysis | Rattus norvegicus | 2 | ALA5137073 | organism-based format | Scientific Literature | |
12. | ALA5141597 | Toxicity | Antiproliferative activity against human PASMC assessed as PDGF-induced reduction cell proliferation incubated for 24 hrs by BrdU Cell Proliferation assay | Homo sapiens | 3 | ALA5137073 | cell-based format | Scientific Literature | |
13. | ALA5141598 | Binding | Activation of cGMP induced partially activated state recombinant human full length N-terminal His tagged PKG1beta expressed in Baculovirus infected Sf9 cell expression system using Glass-tide peptide substrate incubated for 30 mins followed by ATP addition and measured after 1 hr in presence of cGMP by ADP-GLO Max Assay | Homo sapiens | 3 | ALA5137073 | cell-based format | Scientific Literature | |
14. | ALA5141599 | Binding | Binding affinity to human PKG1alpha cGMP-binding site assessed as change in deuteration at 20 to 800 uM incubated for 30 mins at room temperature and overnight at 4 degreeC by HDX-MS analysis | Homo sapiens | 2 | ALA5137073 | single protein format | Scientific Literature |