# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5136283 | Functional | Antiproliferative activity against human HCC827 cells assessed as cell growth inhibition incubated for 3 days by CCK-8 assay | Homo sapiens | 16 | ALA5131542 | cell-based format | Scientific Literature | |
2. | ALA5136302 | Binding | PROTAC activity at CRBN/wild type EGFR overexpressed in human OVCAR-8 cells assessed as EGFR degradation upto 10000 nM treated after 8 hrs under serum starvation condition and preincubated with 1 to 5 uM pictilisib measured for 2 hrs after 16 hrs of compound addition by Western blotting analysis | Homo sapiens | 1 | ALA5131542 | cell-based format | Scientific Literature | |
3. | ALA5136308 | Binding | Binding affinity to CRBN/EGFR mutant in human HCC827 cell lysate assessed as ternary complex formation by measuring EGFR pulldown at 100 nM measured after 16 hrs by CRBN antibody based co-immunoprecipitation assay | Homo sapiens | 1 | ALA5131542 | subcellular format | Scientific Literature | |
4. | ALA5136313 | Binding | Binding affinity to CRBN/wild type EGFR in human OVCAR-8 cell lysate assessed as ternary complex formation by measuring CRBN pulldown at 1 uM pretreated with pictilisib for 2 hrs followed by compound addition measured after 16 hrs by EGFR antibody based co-immunoprecipitation assay | Homo sapiens | 1 | ALA5131542 | subcellular format | Scientific Literature | |
5. | ALA5136314 | Binding | Binding affinity to CRBN/wild type EGFR in human NCI-H1299 cell lysate assessed as ternary complex formation by measuring CRBN pulldown at 1 uM pretreated with pictilisib for 2 hrs followed by compound addition measured after 16 hrs by EGFR antibody based co-immunoprecipitation assay | Homo sapiens | 1 | ALA5131542 | subcellular format | Scientific Literature | |
6. | ALA5136317 | Binding | Binding affinity to CRBN/wild type EGFR in human OVCAR-8 cell lysate assessed as ternary complex formation by measuring EGFR pulldown at 1 uM pretreated with pictilisib for 2 hrs followed by compound addition measured after 16 hrs by CRBN antibody based co-immunoprecipitation assay | Homo sapiens | 1 | ALA5131542 | subcellular format | Scientific Literature | |
7. | ALA5136318 | Binding | Binding affinity to CRBN/wild type EGFR in human NCI-H1299 cell lysate assessed as ternary complex formation by measuring EGFR pulldown at 1 uM pretreated with pictilisib for 2 hrs followed by compound addition measured after 16 hrs by CRBN antibody based co-immunoprecipitation assay | Homo sapiens | 1 | ALA5131542 | subcellular format | Scientific Literature | |
8. | ALA5136319 | Functional | Antiproliferative activity against human OVCAR-8 cells expressing wild type EGFR assessed as cell growth inhibition at 1 uM in presence of pictilisib | Homo sapiens | 4 | ALA5131542 | cell-based format | Scientific Literature | |
9. | ALA5136320 | Functional | Antiproliferative activity against human NCI-H1299 cells expressing wild type EGFR assessed as cell growth inhibition at 1 uM in presence of pictilisib | Homo sapiens | 4 | ALA5131542 | cell-based format | Scientific Literature |