#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5166476	B	Displacement of [3H]HOCPCA from CaMK2alpha in rat brain cerebral cortex membrane homogenates assessed as inhibition constant measured after 60 mins by TopCount scintillation counting method	Rattus norvegicus	4		cell membrane format	Scientific Literature
2.	ALA5166478	B	Displacement of [3H]NCS-382 from CaMK2alpha in rat brain cerebral cortex membrane homogenates assessed as inhibition constant measured after 60 mins by TopCount scintillation counting method	Rattus norvegicus	21		cell membrane format	Scientific Literature
3.	ALA5166479	B	Displacement of [3H]HOCPCA from recombinant full length CaMK2alpha (unknown origin) expressed in HEK293 cell homogenates assessed as inhibition constant measured after 60 mins by liquid scintillation counting method	Homo sapiens	2		cell-based format	Scientific Literature
4.	ALA5166480	A	Plasma concentration in C57BL/6J mouse at 10 mg/kg , po measured after 30 mins by UPLC based tandem mass spectrometry analysis	Mus musculus	2		organism-based format	Scientific Literature
5.	ALA5166481	B	Binding affinity to recombinant human CaMK2alpha Thr354Asn mutant expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by surface plasmon resonance assay	Homo sapiens	4		assay format	Scientific Literature
6.	ALA5166483	B	Binding affinity to recombinant human CaMK2alpha Glu355Gln mutant expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay	Homo sapiens	4		assay format	Scientific Literature
7.	ALA5166484	B	Binding affinity to recombinant human CaMK2alpha Thr412Asn mutant expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay	Homo sapiens	4		assay format	Scientific Literature
8.	ALA5166485	B	Binding affinity to recombinant human CaMK2alpha Ile414Met mutant expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay	Homo sapiens	4		assay format	Scientific Literature
9.	ALA5166486	B	Binding affinity to recombinant human CaMK2alpha Ile464His mutant expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay	Homo sapiens	4		assay format	Scientific Literature
10.	ALA5166487	B	Binding affinity to recombinant human CaMK2alpha Phe467Met mutant expressed in Escherichia coli BL21 DE3 assessed as dissociation constant by surface plasmon resonance assay	Homo sapiens	4		assay format	Scientific Literature
11.	ALA5166489	B	Binding affinity to recombinant human CaMK2alpha (345 to 475 residues) expressed in Escherichia coli BL21 DE3 assessed as thermal stability by measuring change in melting temperature at 0.625 to 160 uM by SYPRO orange dye based differential scanning fluorimetry	Homo sapiens	1		assay format	Scientific Literature
12.	ALA5166492	P	Chemical stability of compound in PBS buffer at pH 7.4 assessed as cyclized intermediate formation at 25 to 37 degreeC incubated for 7 days by HPLC based LC-MS analysis		1		small-molecule physicochemical format	Scientific Literature
13.	ALA5166493	P	Chemical stability of compound in PBS buffer at pH 7.4 assessed as oxidized intermediate formation at 25 to 37 degreeC incubated for 7 days by HPLC based LC-MS analysis		1		small-molecule physicochemical format	Scientific Literature
14.	ALA5166494	P	Glutathione reactivity of the compound assessed as GSH-adduct formation at 0.5 mg/ml measured after 4 hrs in absence of CYP enzymes and NADPH by HPLC based LC-MS analysis		1		small-molecule physicochemical format	Scientific Literature
15.	ALA5166497	A	Brain concentration in C57BL/6J mouse at 10 mg/kg , po measured after 30 mins by UPLC based tandem mass spectrometry analysis	Mus musculus	2		organism-based format	Scientific Literature
16.	ALA5166499	A	Ratio of drug concentration in brain to plasma in C57BL/6J mouse at 10 mg/kg , po measured after 30 mins by UPLC based tandem mass spectrometry analysis	Mus musculus	2		organism-based format	Scientific Literature
17.	ALA5166516	A	Drug metabolism in PBS buffer at pH 7.4 assessed as S-conjugation of parent compound with 2-mercaptoethanol at 0.5 mg/ml measured after 4 hrs by LC-MS/MS analysis		1		assay format	Scientific Literature