# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5108411 | B | Inhibition of GST-tagged recombinant UCHL1 (unknown origin) using Ub-Lys-TAMRA as substrate incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 2 | ALA5107948 | single protein format | Scientific Literature | |
2. | ALA5108412 | B | Inhibition of GST-tagged recombinant UCHL3 (unknown origin) using Ub-Lys-TAMRA as substrate incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 2 | ALA5107948 | single protein format | Scientific Literature | |
3. | ALA5108413 | B | Inhibition of GST-tagged recombinant UCHL1 (unknown origin) using Ub-Lys-TAMRA as substrate at 1 uM incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 2 | ALA5107948 | single protein format | Scientific Literature | |
4. | ALA5108414 | B | Inhibition of GST-tagged recombinant USP30 (unknown origin) using Ub-Lys-TAMRA as substrate at 1 uM incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 2 | ALA5107948 | single protein format | Scientific Literature | |
5. | ALA5108415 | B | Binding affinity to GST-tagged recombinant UCHL1 (unknown origin) assessed as covalent adduct formation at 13 uM incubated for 1 hr by LC-ESI-MS analysis | Homo sapiens | 2 | ALA5107948 | single protein format | Scientific Literature | |
6. | ALA5108416 | T | Cytotoxicity against human HEK293 cells assessed as reduction in cell growth incubated for 72 hrs by live-cell time-lapse imaging | Homo sapiens | 2 | ALA5107948 | cell-based format | Scientific Literature | |
7. | ALA5108417 | B | Inhibition of FLAG-tagged UCHL1 (unknown origin) expressed in CAL-51 cells using HA-Ub-VME as substrate incubated for 30 mins by HTRF assay | Homo sapiens | 2 | ALA5107948 | cell-based format | Scientific Literature | |
8. | ALA5108418 | B | Inhibition of UCHL1 in human HEK293 cells assessed as reduction in UCHL1-Ub level using HA-Ub-VME as substrate at 0.001 to 30 uM incubated for 1 hr by immunoblotting analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
9. | ALA5108419 | B | Inhibition of UCHL1 in human HEK293 cells assessed as reduction in UCHL1 expression level using HA-Ub-VME as substrate at 0.001 to 30 uM incubated for 1 hr by immunoblotting analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
10. | ALA5108420 | B | Inhibition of UCHL1 in human HEK293 cells using HA-Ub-VME as substrate at 0.1 to 10 uM incubated for 1 hr by immunoblotting analysis | Homo sapiens | 2 | ALA5107948 | cell-based format | Scientific Literature | |
11. | ALA5108421 | B | Inhibition of UCHL3 in human HEK293 cells using HA-Ub-VME as substrate at 0.1 to 10 uM incubated for 1 hr by immunoblotting analysis | Homo sapiens | 2 | ALA5107948 | cell-based format | Scientific Literature | |
12. | ALA5108422 | B | Inhibition of UCHL5 in human HEK293 cells using HA-Ub-VME as substrate at 0.1 to 10 uM incubated for 1 hr by immunoblotting analysis | Homo sapiens | 2 | ALA5107948 | cell-based format | Scientific Literature | |
13. | ALA5108423 | B | Inhibition of BAP1 in human HEK293 cells using HA-Ub-VME as substrate at 0.1 to 10 uM incubated for 1 hr by immunoblotting analysis | Homo sapiens | 2 | ALA5107948 | cell-based format | Scientific Literature | |
14. | ALA5108424 | B | Binding affinity to UCHL1 in human HEK293 cells at 40 nM incubated for 3 hrs by In-gel fluorescence analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
15. | ALA5108425 | B | Binding affinity to UCHL1 in human HEK293 cells at 31 nM by TAMRA labelled pulldown based In-gel fluorescence analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
16. | ALA5108426 | B | Binding affinity to UCHL1 in human HEK293 cells at 31 nM by TAMRA labelled pulldown based immunoblotting analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
17. | ALA5108427 | B | Binding affinity to UCHL1 in human HEK293 cells at 250 nM incubated for 6 hrs in presence of BME by TAMRA labelled In-gel fluorescence analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
18. | ALA5108429 | B | Binding affinity to UCHL1 in human HEK293 cells assessed as fold change at 130 nM incubated for 3 to 6 hrs by LFQ-chemical proteomics analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
19. | ALA5108430 | B | Binding affinity to ALDH9A1 in human HEK293 cells assessed as fold change at 130 nM incubated for 3 to 6 hrs by LFQ-chemical proteomics analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature | |
20. | ALA5108431 | B | Binding affinity to ALDH2 in human HEK293 cells assessed as fold change at 130 nM incubated for 3 to 6 hrs by LFQ-chemical proteomics analysis | Homo sapiens | 1 | ALA5107948 | cell-based format | Scientific Literature |