# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5125818 | B | Inhibition of C-terminal domain of human sEH-H assessed as reduction in fluorescent naphthalene aldehyde formation using PHOME as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured every minute for 45 mins by fluorescence based analysis | Homo sapiens | 5 | ALA5120942 | single protein format | Scientific Literature | |
2. | ALA5125819 | B | Inhibition of C-terminal domain of mouse sEH-H assessed as reduction in fluorescent naphthalene aldehyde formation using PHOME as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured every minute for 45 mins by fluorescence based analysis | Mus musculus | 1 | ALA5120942 | single protein format | Scientific Literature | |
3. | ALA5125820 | B | Binding affinity to C-terminal domain of human sEH-H at 20 nM incubated for 1 hr by fluorescence polarization assay | Homo sapiens | 1 | ALA5120942 | single protein format | Scientific Literature | |
4. | ALA5125821 | B | Inhibition of lipid phosphatase activity of N-terminal domain of human sEH | Homo sapiens | 1 | ALA5120942 | single protein format | Scientific Literature | |
5. | ALA5125822 | B | Inhibition of lipid phosphatase activity of N-terminal domain of mouse sEH | Mus musculus | 1 | ALA5120942 | single protein format | Scientific Literature | |
6. | ALA5125824 | B | Binding affinity to NanoLuc-fused human sEH ( 1 to 555 residues) expressed in HEK293T cells incubated for 5 hrs by NanoBRET analysis | Homo sapiens | 1 | ALA5120942 | cell-based format | Scientific Literature | |
7. | ALA5125825 | B | Binding affinity to NanoLuc-fused human sEH ( 1 to 555 residues) expressed in HEK293T cells incubated for 5 hrs in presence of (1R,3S)-N-(4-cyano-2-(trifluoromethyl)benzyl)-3-(4-methyl-6-(methylamino)-1,3,5-triazin-2-ylamino)cyclohexanecarboxamide by NanoBRET analysis | Homo sapiens | 1 | ALA5120942 | cell-based format | Scientific Literature | |
8. | ALA5125827 | B | Binding affinity to human full length sEH (1 to 555 residues) expressed in doxycycline-induced HEK293 cells at 300 nM incubated for 5 hrs by Hoechst 33342 staining based fluorescence microscopy | Homo sapiens | 1 | ALA5120942 | cell-based format | Scientific Literature | |
9. | ALA5125828 | B | Binding affinity to sEH in C57BL/6J mouse astrocytes at 10 uM incubated for 5 hrs by DAPI staining based immunocytochemistry | Mus musculus | 1 | ALA5120942 | cell-based format | Scientific Literature |