Binding affinity to human galectin-1 using 3,3'-dideoxy-3-[4-(fluorescein-5-yl-carbonylaminomethyl)-1H-1,2,3-triazol-1-y1]-3'.(3,5-dimethoxy-benzamido)-1,1'-sulfanediyl-di-beta-D-galactopyranoside as fluorescent probe by fluorescence polarization analysis
Binding affinity to human galectin-3 using 2-(fluorescein-5/6-yl-carbonylamino)-ethyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside by fluorescence polarization analysis
Binding affinity to human galectin-4N expressed in Escherichia coli BL21 using 3,3'-dideoxy-3-[4-(fluorescein-5-yl-carbonylaminomethyl)-1H-1,2,3-triazol-1-y1]-3'-(3,5-dimethoxy-benzamido)-1,1'-sulfanediyl-di-beta-D-galactopyranoside as fluorescent probe by fluorescence polarization analysis
Binding affinity to human galectin-4C using 2-(fluorescein-5/6-yl-carbonylamino)-ethyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranosyl-(1-4)-beta-D-glucopyranoside as fluorescent probe by fluorescence polarization analysis
Binding affinity to human galectin-8C expressed in Escherichia coli BL21(DE3) using 3,3'-dideoxy-3-[4-(fluorescein-5-yl-carbonylaminomethyl)-1H-1,2,3-triazol-1-y1]-3'-(3,5-dimethoxy-benzamido)-1,1'-sulfanediyl-di-beta-D-galactopyranoside as fluorescent probe by fluorescence polarization analysis
Cytotoxicity against human US7 patient-derived BCP-ALL cells co-cultured with irradiated OP9 stromal cells assessed as reduction in cell viability incubated for 24 hrs by Alamar blue assay
Cytotoxicity against human LAX40 patient-derived BCP-ALL cells co-cultured with irradiated OP9 stromal cells assessed as reduction in cell viability at 100 uM for 1 to 3 days by trypan blue staining based assay
Cytostatic activity against human LAX40 patient-derived BCP-ALL cells co-cultured with irradiated OP9 stromal cells assessed as reduction in cell viability at 100 uM for 1 to 3 days by trypan blue staining based assay