# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5256457 | B | PROTAC activity at CRBN/H-PGDS in human KU812 cells assessed as induction of H-PGDS degradation by measuring decrease in H-PGDS protein expression incubated for 24 hrs by western blot analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
2. | ALA5256458 | P | Solubility of the compound in PBS buffer at 100 uM at pH 7.4 by UPLC/MS/MS analysis | 8 | small-molecule physicochemical format | Scientific Literature | |||
3. | ALA5256459 | P | Lipophilicity, logD of the compound by UPLC analysis | 8 | small-molecule physicochemical format | Scientific Literature | |||
4. | ALA5256460 | A | Apparent permeability coefficient, Papp of the compound at pH 7.4 incubated for 4 hrs by UPLC/MS/MS analysis | 8 | assay format | Scientific Literature | |||
5. | ALA5256461 | A | Metabolic stability of the compound in human liver microsomes incubated for 10 mins in presence of NADPH by UPLC/MS/MS analysis | Homo sapiens | 8 | microsome format | Scientific Literature | ||
6. | ALA5256462 | A | Metabolic stability of the compound in human liver microsomes incubated for 60 mins in presence of NADPH by UPLC/MS/MS analysis | Homo sapiens | 8 | microsome format | Scientific Literature | ||
7. | ALA5113356 | B | Binding affinity to recombinant human H-PGDS incubated for 1 hrs by competitive fluorescence polarization assay | Homo sapiens | 6 | single protein format | Scientific Literature | ||
8. | ALA5113357 | B | PROTAC activity at CRBN/H-PGDS in human KU812 cells assessed as reduction in H-PGDS levels incubated for 24 hrs by Western blotting analysis | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
9. | ALA5113363 | B | PROTAC activity at CRBN/H-PGDS in human KU812 cells assessed as reduction in H-PGDS levels at 10 nM incubated for 6 hrs in the presence of MG132 by Western blotting analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA5113364 | B | PROTAC activity at CRBN/H-PGDS in human KU812 cells assessed as reduction in H-PGDS levels at 10 nM incubated for 6 hrs in the presence of MLN7243 by Western blotting analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
11. | ALA5113365 | B | PROTAC activity at CRBN/H-PGDS in human KU812 cells assessed as reduction in H-PGDS levels at 10 nM incubated for 6 hrs in the presence of TAS-205 by Western blotting analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
12. | ALA5113366 | B | PROTAC activity at CRBN/H-PGDS in human KU812 cells assessed as reduction in A23187-stimulated PGD2 levels at 10 nM preincubated for 24 hrs followed by A23187 stimulation for 30 mins by ELISA analysis relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature |