| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5121010 | B | Inhibition of recombinant human N-terminal His6-SUMO-tagged C-terminal SII-tagged HDAC4 expressed in Escherichia coli (BL21) DE3 pLysS cells assessed as residual activity at 50 uM using BocLys(trifluoroacetyl)-AMC as substrate incubated for 30 mins by fluorescence assay relative to control | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 2. | ALA5121011 | B | Inhibition of recombinant human N-terminal His6-SUMO-tagged C-terminal SII-tagged HDAC8 expressed in Escherichia coli (BL21) DE3 pLysS cells assessed as residual activity at 50 uM using BocLys(trifluoroacetyl)-AMC as substrate incubated for 30 mins by fluorescence assay relative to control | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 3. | ALA5121012 | B | Inhibition of recombinant human N-terminal His6-SUMO-tagged C-terminal SII-tagged HDAC4 expressed in Escherichia coli (BL21) DE3 pLysS cells using BocLys(trifluoroacetyl)-AMC as substrate incubated for 30 mins by fluorescence assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 4. | ALA5121013 | B | Inhibition of recombinant human N-terminal His6-SUMO-tagged C-terminal SII-tagged HDAC8 expressed in Escherichia coli (BL21) DE3 pLysS cells using BocLys(trifluoroacetyl)-AMC as substrate incubated for 30 mins by fluorescence assay | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
| 5. | ALA5121014 | B | Inhibition of recombinant human C-terminal His6-FLAG-tagged HDAC1 (1 to end residues) expressed in sf9 insect cells using Boc-Lys(acetyl)-AMC as substrate incubated for 30 mins by fluorescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 6. | ALA5121015 | B | Inhibition of recombinant human HDAC2 Boc-Lys(acetyl)-AMC as substrate incubated for 30 mins by fluorescence assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 7. | ALA5121016 | B | Inhibition of C-terminal His-tagged recombinant human HDAC3 (1 to 428 residues)/N-terminal GST-tagged recombinant human NCoR2 (395 to 489 residues) co-expressed in baculovirus infected Sf9 cells using Boc-Lys(acetyl)-AMC as substrate incubated for 30 mins by fluorescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 8. | ALA5121017 | B | Inhibition of recombinant human HDAC6 using Boc-Lys(acetyl)-AMC as substrate incubated for 30 mins by fluorescence assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 9. | ALA5121018 | B | Inhibition of recombinant human N-terminal GST-tagged HDAC7 (518 to end residues) expressed in baculovirus infected sf9 insect cells using BocLys(trifluoroacetyl)-AMC as substrate incubated for 30 mins by fluorescence assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 10. | ALA5121020 | B | Binding affinity to recombinant human N-terminal His6-SUMO-tagged C-terminal SII-tagged HDAC4 expressed in Escherichia coli (BL21) DE3 pLysS cells assessed as melting temperature at 100 uM for 15 mins by thermal shift assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA5121021 | T | Cytotoxicity against HUVEC cells assessed as reduction in cell viability after 96 hrs by MTT assay | Homo sapiens | 10 | cell-based format | Scientific Literature |
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