Antiviral activity against pseudovirus SARS-CoV-2 spike protein infected human HEK293T cells overexpressing ACE2 assessed as inhibition on pseudovirus infection at 10 uM incubated for 48 hrs by luciferase assay
Antiviral activity against pseudovirus SARS-CoV-2 spike protein infected human HEK293T cells overexpressing ACE2 assessed as inhibition on pseudovirus infection at 40 uM incubated for 48 hrs by luciferase assay
Antiviral activity against pseudovirus SARS-CoV-2 spike protein infected human HEK293T cells overexpressing ACE2 assessed as inhibition on pseudovirus infection incubated for 48 hrs by luciferase assay
Antiviral activity against SARS-CoV-2 VSV-G pseudotyped virus infected human HEK293T cells overexpressing ACE2 incubated for 48 hrs by luciferase assay
Selectivity index, ratio of CC50 for cytotoxicity against human HEK293T cells overexpressing human ACE2 to EC50 for antiviral activity against SARS-CoV-2 VSV-G pseudotyped virus infected human HEK293T cells overexpressing ACE2
Antiviral activity against SARS-CoV-2 Wuhan-HU-1 infected African green monkey Vero E6 cells preincubated for 1 hrs followed by incubation with virus 1 hr and measured after 24 hrs by fluorescence assay
Selectivity index, ratio of CC50 for cytotoxicity against African green monkey Vero E6 cells to EC50 for antiviral activity against SARS-CoV-2 Wuhan-Hu-1 infected African green monkey Vero E6 cells
Antiviral activity against SARS-CoV-2 Wuhan-HU-1 infected African green monkey Vero E6 cells assessed as reduction in N-protein expression at 0.55 to 15 uM by indirect immunofluorescence assay
Antiviral activity against pseudovirus SARS-CoV-2 spike protein with E484K mutant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on pseudovirus infection at 5 to 40 uM incubated for 48 hrs by luciferase assay
Antiviral activity against pseudovirus SARS-CoV-2 spike protein with D614G mutant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on pseudovirus infection at 5 to 40 uM incubated for 48 hrs by luciferase assay
Antiviral activity against pseudovirus SARS-CoV-2 spike protein with N501Y mutant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on pseudovirus infection at 5 to 40 uM incubated for 48 hrs by luciferase assay
Antiviral activity against pseudotyped spike protein mutant B.1.1.7 SARS-CoV-2 Alpha variant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on viral entry incubated for 48 hrs by luciferase assay
Antiviral activity against pseudotyped spike protein mutant B.1.351 SARS-CoV-2 Beta variant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on viral entry incubated for 48 hrs by luciferase assay
Antiviral activity against pseudotyped spike protein mutant P.10 SARS-CoV-2 Gamma variant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on viral entry incubated for 48 hrs by luciferase assay
Antiviral activity against pseudovirus spike protein mutant B.1.617.2 SARS-CoV-2 Delta variant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on viral entry incubated for 48 hrs by luciferase assay
Antiviral activity against pseudovirus SARS-CoV-2 spike protein mutant Omicron variant infected human HEK293T cells overexpressing ACE2 assessed as inhibition on viral entry incubated for 48 hrs by luciferase assay
Binding affinity to SARS-CoV-2 Spike protein S1 subunit assessed as blockage of receptor-binding domain S1 subunit interaction with human ACE2 in human HEK293T cells by Co-IP/western blot analysis