# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5098726 | Binding | Binding affinity to N-terminal His-tagged wild type ML-IAP (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as change in melting temperature at 20 uM incubated for 2 hrs followed by heating at 40 to 70 degree C for 30 mins by SYPRO orange dye based thermal shift assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
2. | ALA5098727 | Binding | Antagonist activity at N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
3. | ALA5098728 | Binding | Antagonist activity at N-terminal His-tagged XIAP-BIR3 domain (253 to 347 residues)(unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
4. | ALA5098729 | Binding | Antagonist activity at N-terminal His-tagged cIAP1-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
5. | ALA5098730 | Binding | Antagonist activity at N-terminal His-tagged cIAP2-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate incubated for 2 hrs by DELFIA assay | Homo sapiens | 12 | ALA5096205 | assay format | Scientific Literature | |
6. | ALA5098731 | Binding | Antagonist activity at N-terminal His-tagged wild type ML-IAP BIR domain (63 to 179 residues)(unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
7. | ALA5098732 | Binding | Antagonist activity at N-terminal His-tagged XIAP-BIR3 domain (253 to 347 residues)(unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
8. | ALA5098733 | Binding | Antagonist activity at N-terminal His-tagged cIAP1-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
9. | ALA5098734 | Binding | Antagonist activity at N-terminal His-tagged cIAP2-BIR3 domain (unknown origin) expressed in Escherichia coli BL21 using AVPIAQKSEK-biotinylated peptide as substrate preincubated for 2 hrs with protein followed by substrate addition measured after 2 hrs by DELFIA assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
10. | ALA5098742 | Binding | Binding affinity to N-terminal His-tagged ML-IAP K135A mutant (63 to 179 residues) (unknown origin) expressed in Escherichia coli BL21 assessed as change in melting temperature at 20 uM incubated for 2 hrs followed by heating at 40 to 70 degree C for 30 mins by SYPRO orange dye based thermal shift assay | Homo sapiens | 3 | ALA5096205 | assay format | Scientific Literature | |
11. | ALA5098743 | Binding | Binding affinity to ML-IAP in human SK-MEL-28 cells assessed as change in melting temperature at 10 uM incubated for 3 hrs followed by heating at 37 to 70 degree C for 3 mins by CETSA analysis (Rvb = 50 degreeC) | Homo sapiens | 3 | ALA5096205 | cell-based format | Scientific Literature | |
12. | ALA5098746 | Functional | Induction of apoptosis in human SK-MEL-28 cells assessed as increase in caspase-3/7 activity measured up to 48 hrs in presence of 0.1 ng/mL TNFlpha by IncuCyteS3 caspase-3/7 fluorescence-based apoptosis assay | Homo sapiens | 3 | ALA5096205 | cell-based format | Scientific Literature | |
13. | ALA5098747 | Functional | Induction of apoptosis in human SK-MEL-28 cells assessed as increase in caspase-3/7 activity at 0.1 to 10 uM in presence of 0.1 ng/mL TNFlpha by IncuCyteS3 caspase-3/7 fluorescence-based apoptosis assay | Homo sapiens | 3 | ALA5096205 | cell-based format | Scientific Literature |