Binding affinity to human N-terminal His-tagged/ TEV cleavage fused DRAK1 (39 to 369 residues) expressed in Escherichia coli assessed as change in melting temperature at 10 uM by SYPRO orange dye based DSF assay
Binding affinity to wild type full length human CK2alpha1 (1 to 391 residues) expressed in mammalian expression system assessed as change in melting temperature at 10 uM by SYPRO orange dye based DSF assay
Binding affinity to wild type full length human CK2alpha2 (1 to 350 residues) expressed in mammalian expression system assessed as change in melting temperature by SYPRO orange dye based DSF assay
Binding affinity to wild type full length human DRAK2 (1 to 372 residues) expressed in bacterial expression system assessed as change in melting temperature by SYPRO orange dye based DSF assay
Inhibition of human N-terminal His-tagged/ TEV cleavage fused DRAK1 (39 to 369 residues) transfected in HEK293T cells using NanoBRET NanoGlo as substrate incubated for 2 hrs by NanoBRET assay