#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5169003	B	Binding affinity to NLuc-tagged human H1R expressed in HEK293T cells using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	Homo sapiens	30		cell-based format	Scientific Literature
2.	ALA5169007	B	Binding affinity to human H1R expressed in CHO cells using Fluo-4AM as substrate incubated for 1 hrs by calcium mobilization assay based fluorometric analysis	Homo sapiens	10		cell-based format	Scientific Literature
3.	ALA5169008	B	Binding affinity to NLuc-tagged human H3R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	Homo sapiens	3		cell-based format	Scientific Literature
4.	ALA5169009	B	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	Homo sapiens	3		cell-based format	Scientific Literature
5.	ALA5169010	B	Binding affinity to NLuc-tagged human H1R expressed in HEK293T cells assessed as association rate constant (kon) using furimazine as substrate measured after 90 mins by NanoBRET binding assay	Homo sapiens	2		cell-based format	Scientific Literature
6.	ALA5169011	B	Binding affinity to NLuc-tagged human H1R expressed in HEK293T cells assessed as dissociation rate constant (koff) using furimazine as substrate measured after 90 mins by NanoBRET binding assay	Homo sapiens	2		cell-based format	Scientific Literature
7.	ALA5169012	B	Binding affinity to NLuc-tagged human H1R expressed in HEK293T cells assessed as mean residence time (1/koff) using furimazine as substrate measured after 90 mins by NanoBRET binding assay	Homo sapiens	2		cell-based format	Scientific Literature
8.	ALA5169013	B	Binding affinity to NLuc-tagged human H1R expressed in HEK293T cells at assessed as ratio of association rate constant to dissociation rate constant by NanoBRET binding assay	Homo sapiens	2		cell-based format	Scientific Literature
9.	ALA5169015	B	Binding affinity to NLuc-tagged human H3R expressed in HEK293T cell homogenates at 1 uM using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min in presence of clobenpropit by NanoBRET saturation binding assay	Homo sapiens	3		cell-based format	Scientific Literature
10.	ALA5169016	B	Binding affinity to human H1R expressed in CHO cells assessed as shift in histamine-mediated calcium response at 1 uM using Fluo-4AM as a substrate incubated for 1 hrs by calcium mobilization assay based fluorometric analysis	Homo sapiens	10		cell-based format	Scientific Literature
11.	ALA5169017	B	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates at 1 uM using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min in presence of clobenpropit by NanoBRET saturation binding assay	Homo sapiens	3		cell-based format	Scientific Literature