#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5100431	B	Positive allosteric modulator activity in human A3AR opioid receptor stably expressed in human HEK293 cell membrane assessed as decrease in radioligand [125I]I-AB-MECA dissociation from A3AR receptor by measuring remaining radioligand-bound receptor at 10 uM preincubated for 2 hrs followed by AR agonist NECA addition and measured after 1 hr by radioligand competition binding assay relative to control (Rvb= 26%)	Homo sapiens	27		cell membrane format	Scientific Literature
2.	ALA5100432	B	Positive allosteric modulator activity in human A3AR opioid receptor stably expressed in human HEK293 cell membrane assessed as net change in radioligand [125I]I-AB-MECA equilibrium binding to A3AR receptor at 10 uM incubated for 18 hrs by radioligand competition binding assay relative to control	Homo sapiens	27		cell membrane format	Scientific Literature
3.	ALA5100433	B	Positive allosteric modulator activity in wild type human A3AR opioid receptor stably expressed in human HEK293 cell membrane assessed as increase in CI-IB-MECA induced [35S]GTPGAgammaS binding to receptor at 1 uM preincubated for 1 hrs with CI-IB-MECA further incubated with [35S]GTPGAgammaS for 2 hrs in presence of PSB-603,ZM241385 and ADP to degrade endogenous AR by liquid scintillation spectrometry	Homo sapiens	32		cell membrane format	Scientific Literature
4.	ALA5100435	B	Positive allosteric modulator activity in wild type human A3AR opioid receptor stably expressed in human HEK293 cell membrane assessed as increase in CI-IB-MECA induced [35S]GTPGAgammaS binding to receptor at 0.1 uM preincubated for 1 hrs with CI-IB-MECA further incubated with [35S]GTPGAgammaS for 2 hrs in presence of PSB-603,ZM241385 and ADP to degrade endogenous AR by liquid scintillation spectrometry	Homo sapiens	4		cell membrane format	Scientific Literature