| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5166602 | B | Binding affinity to N-terminal 6-His tagged human LRH-1 LBD (299 to 541 residues) expressed in Escherichia coli BL21-(DE3) assessed as inhibition constant using 6N-FAM as substrate by fluorescence polarization competition assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 2. | ALA5166603 | B | Agonist activity at LRH-1 (unknown origin) transfected in human HeLa cells measured after 24 hrs by Dual Glo luciferase reporter gene assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 3. | ALA5166604 | B | Agonist activity at LRH-1 (unknown origin) transfected in human HeLa cells assessed as fold activation incubated for 24 hrs by DualGlo luciferase reporter gene assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 4. | ALA5166605 | B | Binding affinity to N-terminal 6-His tagged human LRH-1 LBD (299 to 541 residues) expressed in Escherichia coli BL21-(DE3) assessed as thermal stability by measuring melting temperature incubated overnight by fluorescence based assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 5. | ALA5166610 | B | Agonist activity at LRH-1 in human HepG2 cells assessed as downregulation of Shp mRNA expression at 10 uM incubated for 24 hrs by RT-qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA5166611 | B | Agonist activity at LRH-1 in human HepG2 cells assessed as downregulation of Cyp7a1 mRNA expression at 10 uM incubated for 24 hrs by RT-qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA5166774 | B | Binding affinity to N-terminal 6-His tagged human LRH-1 LBD (299 to 541 residues) expressed in Escherichia coli BL21-(DE3) assessed as dissociation constant of SRC3 using FAM-labeled +H3N-KKENNALLRYLLDRDDPSD-CO2- peptide as substrate incubated overnight followed by substrate addition incubated for overnight by fluorescence polarization assay (Rvb = 7 microM) | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 8. | ALA5166775 | B | Binding affinity to N-terminal 6-His tagged human LRH-1 LBD (299 to 541 residues) expressed in Escherichia coli BL21-(DE3) assessed as dissociation constant of PGC-1alpha using FAM-labeled +H3N-EAEEPSLLKKLLLAPANTQ-CO2- peptide as substrate incubated overnight followed by substrate addition incubated for overnight by fluorescence polarization assay (Rvb = 1300 nM) | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 9. | ALA5166776 | B | Binding affinity to N-terminal 6-His tagged human LRH-1 LBD (299 to 541 residues) expressed in Escherichia coli BL21-(DE3) assessed as dissociation constant of SHP using FAM-labeled +H3N-QGAASRPAILYALLSSSLK-CO2- peptide as substrate incubated overnight followed by substrate addition incubated for overnight by fluorescence polarization assay (Rvb = 1200 nM) | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 10. | ALA5166777 | B | Agonist activity to full length LRH-1 (unknown origin) transfected in human HeLa cells co-transfected with NCOR1 assessed as minimal effect of NCOR1 at 0.1 to 10 uM incubated for 24 hrs by DualGlo luciferase reporter assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA5166783 | B | Agonist activity to full length LRH-1 (unknown origin) transfected in human HeLa cells co-transfected with PGC-1 assessed as effect on PGC-1 expression at 0.1 to 10 uM incubated for 24 hrs by DualGlo luciferase reporter assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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