#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5148453	B	Inhibition of recombinant Trypanosoma brucei PTR1 expressed in Escherichia coli BL21 (DE3) cells using H2B as substrate in presence of cytochrome c and NADPH by envision multilabel reader	Trypanosoma brucei	34		cell-based format	Scientific Literature
2.	ALA5148454	B	Inhibition of recombinant Leishmania major PTR1 expressed in Escherichia coli BL21 (DE3) cells using H2B as substrate in presence of cytochrome c and NADPH by envision multilabel reader	Leishmania major	34		cell-based format	Scientific Literature
3.	ALA5148455	B	Inhibition of recombinant Trypanosoma brucei DHFR-TS expressed in Escherichia coli BL21 (DE3) cells using dihydrofolate as substrate in presence of NADPH by spectrophotometric analysis	Trypanosoma brucei	34		cell-based format	Scientific Literature
4.	ALA5148456	B	Inhibition of recombinant Leishmania major DHFR-TS expressed in Escherichia coli BL21 (DE3) cells using dihydrofolate as substrate in presence of NADPH by spectrophotometric analysis	Leishmania major	34		cell-based format	Scientific Literature
5.	ALA5148457	T	Inhibition of recombinant human DHFR expressed in Escherichia coli BL21 (DE3) cells using dihydrofolate as substrate in presence of NADPH by spectrophotometric analysis	Homo sapiens	34		cell-based format	Scientific Literature
6.	ALA5148458	T	Selectivity index, ratio of IC50 for human DHFR to IC50 for Trypanosoma brucei PTR1		34		assay format	Scientific Literature
7.	ALA5148459	T	Selectivity index, ratio of IC50 for human DHFR to IC50 for Leishmania major PTR1		33		assay format	Scientific Literature
8.	ALA5148460	T	Selectivity index, ratio of IC50 for human DHFR to IC50 for Trypanosoma brucei DHFR		34		assay format	Scientific Literature
9.	ALA5148461	T	Selectivity index, ratio of IC50 for human DHFR to IC50 for Leishmania major DHFR		34		assay format	Scientific Literature
10.	ALA5148462	F	Antitrypanosomal activity against bloodstream form of Trypanosoma brucei brucei Lister 427 assessed as parasite growth inhibition at 10 uM incubated for 72 hrs by resazurin dye based analysis relative to control	Trypanosoma brucei brucei	7		organism-based format	Scientific Literature
11.	ALA5148463	F	Antitrypanosomal activity against bloodstream form of Trypanosoma brucei brucei Lister 427 assessed as parasite growth inhibition incubated for 72 hrs by resazurin dye based analysis	Trypanosoma brucei brucei	7		organism-based format	Scientific Literature
12.	ALA5148464	T	Cytotoxicity against human THP-1 derived macrophages by colorimetric MTT assay	Homo sapiens	7		cell-based format	Scientific Literature
13.	ALA5148466	T	Inhibition of hERG potassium channel at 10 uM by fluorescence polarization assay relative to control	Homo sapiens	33		assay format	Scientific Literature
14.	ALA5148467	A	Inhibition of CYP1A2 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control	Homo sapiens	33		cell-based format	Scientific Literature
15.	ALA5148468	A	Inhibition of CYP2C9 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control	Homo sapiens	33		cell-based format	Scientific Literature
16.	ALA5148469	A	Inhibition of CYP2C19 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control	Homo sapiens	33		cell-based format	Scientific Literature
17.	ALA5148470	A	Inhibition of CYP2D6 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control	Homo sapiens	33		cell-based format	Scientific Literature
18.	ALA5148471	A	Inhibition of CYP3A4 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control	Homo sapiens	33		cell-based format	Scientific Literature
19.	ALA5148472	T	Mitochondrial toxicity in human 786-0 cells at 10 uM incubated with compound for 6 hrs followed by incubation with MitoTracker Red chloromethyl-X-rosamine dye for 45 mins by imaging analysis relative to control	Homo sapiens	33		cell-based format	Scientific Literature
20.	ALA5148473	T	Cytotoxicity against human A549 cells at 10 uM measured after 48 hrs by Celltiter-Glo assay relative to control	Homo sapiens	33		cell-based format	Scientific Literature