Inhibition of bovine XOD assessed as inhibition of uric acid formation using xanthine as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured for 30 mins by microplate reader analysis
Potency index, ratio of allopurinol IC50 to test compound IC50 for inhibition of bovine XOD assessed as inhibition of uric acid formation using xanthine as substrate preincubated with enzyme for 5 mins followed by substrate addition and measured for 30 mins by microplate reader analysis
Anti-hyperuricemic activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as reduction in serum uric acid level at 20 mg/kg, po administered via gavage once daily for 2 weeks (Rvb = 318.16 +/- 3.31 uM)
Anti-hyperuricemic activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as reduction in serum creatinine level at 20 mg/kg, po administered via gavage once daily for 2 weeks (Rvb = 87.62 +/- 0.67 uM)
Anti-hyperuricemic activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as blood urea nitrogen level at 20 mg/kg, po administered via gavage once daily for 2 weeks (Rvb = 9.88 +/- 1.21 mM)
Nephroprotective activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as reduction in renal tubule dilation at 20 mg/kg, po administered via gavage and measured after 21 days by hematoxylin-eosin and Masson trichrome staining based optical microscopic analysis
Nephroprotective activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as reduction in cytoplasmic vacuolation of renal tubular epithelial cells at 20 mg/kg, po administered via gavage and measured after 21 days by hematoxylin-eosin and Masson trichrome staining based optical microscopic analysis
Nephroprotective activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as appearance of clear tubular structures at 20 mg/kg, po administered via gavage and measured after 21 days by hematoxylin-eosin and Masson trichrome staining based optical microscopic analysis
Nephroprotective activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as reduction in connective tissue hyperplasia at 20 mg/kg, po administered via gavage and measured after 21 days by hematoxylin-eosin and Masson trichrome staining based optical microscopic analysis
Nephroprotective activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as reduction in neutrophils infiltrations at 20 mg/kg, po administered via gavage and measured after 21 days by hematoxylin-eosin and Masson trichrome staining based optical microscopic analysis
Nephroprotective activity against adenine and potassium oxonate-induced hyperuricemia Kunming mouse model assessed as reduction in tubular-interstitial lesion development at 20 mg/kg, po administered via gavage and measured after 21 days by hematoxylin-eosin and Masson trichrome staining based optical microscopic analysis