| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5118572 | B | Inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) using 2-Abz-Ser-AlaVal-Leu-Gln-Ser-Gly-Tyr(3-NO2)-Arg-OH as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins by FRET assay | Severe acute respiratory syndrome coronavirus 2 | 37 | ALA5113504 | assay format | Scientific Literature | |
| 2. | ALA5118573 | B | Inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) using 2-Abz-Ser-AlaVal-Leu-Gln-Ser-Gly-Tyr(3-NO2)-Arg-OH as substrate at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by FRET assay relative to control | Severe acute respiratory syndrome coronavirus 2 | 37 | ALA5113504 | assay format | Scientific Literature | |
| 3. | ALA5118574 | B | Inhibition of full length DENV2 NS2B-NS3 protease in human HeLa cells expressing luciferase reporter gene incubated for 24 hrs by luminescence analysis | dengue virus type 2 | 37 | ALA5113504 | cell-based format | Scientific Literature | |
| 4. | ALA5118575 | B | Inhibition of full length DENV2 NS2B-NS3 protease in human HeLa cells expressing luciferase reporter gene at 12.5 uM incubated for 24 hrs by luminescence analysis relative to control | dengue virus type 2 | 37 | ALA5113504 | cell-based format | Scientific Literature | |
| 5. | ALA5118576 | T | Cytotoxicity against human HeLa cells expressing luciferase-based DENV-2 protease reporter system assessed as reduction in cell viability by celltiter-blue assay | Homo sapiens | 37 | ALA5113504 | cell-based format | Scientific Literature | |
| 6. | ALA5118577 | B | Potency index, ratio of boceprevir IC50 to test compound IC50 for inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) using 2-Abz-Ser-AlaVal-Leu-Gln-Ser-Gly-Tyr(3-NO2)-Arg-OH as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins by FRET assay | Severe acute respiratory syndrome coronavirus 2 | 1 | ALA5113504 | assay format | Scientific Literature | |
| 7. | ALA5118580 | B | Inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) at 50 uM incubated in presence of DTT | Severe acute respiratory syndrome coronavirus 2 | 3 | ALA5113504 | assay format | Scientific Literature | |
| 8. | ALA5118581 | B | Competitive inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) using varying concentrations of 2-Abz-Ser-AlaVal-Leu-Gln-Ser-Gly-Tyr(3-NO2)-Arg-OH as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins by Cheng-prusoff equation analysis | Severe acute respiratory syndrome coronavirus 2 | 2 | ALA5113504 | assay format | Scientific Literature | |
| 9. | ALA5118582 | B | Mixed-type inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) assessed as decrease in Vmax with constant Km using varying concentrations of 2-Abz-Ser-AlaVal-Leu-Gln-Ser-Gly-Tyr(3-NO2)-Arg-OH as substrate by Michaelis-Menten analysis | Severe acute respiratory syndrome coronavirus 2 | 1 | ALA5113504 | assay format | Scientific Literature | |
| 10. | ALA5118583 | B | Inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) assessed as inhibition constant by Michaelis-Menten analysis | Severe acute respiratory syndrome coronavirus 2 | 1 | ALA5113504 | assay format | Scientific Literature | |
| 11. | ALA5118584 | B | Time dependent inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) at 12.5 uM measured upto 60 mins | Severe acute respiratory syndrome coronavirus 2 | 2 | ALA5113504 | assay format | Scientific Literature | |
| 12. | ALA5118585 | B | Reversible inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) at 100 uM preincubated for 2 hrs followed by dilution to 3 uM and substrate addition measured after 15 mins by fluorimetric assay | Severe acute respiratory syndrome coronavirus 2 | 1 | ALA5113504 | assay format | Scientific Literature | |
| 13. | ALA5118586 | B | Binding affinity to C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) assessed as covalent bond formation by measuring mass adduct at 100 uM by ESI-TOF analysis | Severe acute respiratory syndrome coronavirus 2 | 2 | ALA5113504 | assay format | Scientific Literature | |
| 14. | ALA5118588 | B | Inhibition of trypsin (unknown origin) using Boc-Val-Pro-Arg-AMC as substrate assessed as enzymatic cleavage at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by continuous fluorimetric assay | Homo sapiens | 33 | ALA5113504 | protein format | Scientific Literature | |
| 15. | ALA5118589 | B | Inhibition of thrombin (unknown origin) using Boc-Val-Pro-Arg-AMC as substrate assessed as enzymatic cleavage at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by continuous fluorimetric assay | Homo sapiens | 34 | ALA5113504 | single protein format | Scientific Literature | |
| 16. | ALA5118590 | T | Cytotoxicity against human HeLa cells expressing renilla luciferase reporter construct assessed as reduction in cell viability at 12.5 uM by luciferase based analysis | Homo sapiens | 10 | ALA5113504 | cell-based format | Scientific Literature | |
| 17. | ALA5118591 | F | Antiviral activity against Dengue virus serotype 2 infected in Huh-7 cells assessed as reduction in virus titer at 12.5 uM incubated for 48 hrs followed by infection of Vero E6 cells with supernatant and measured after 7 days by crystal violet staining based analysis relative to control | dengue virus type 2 | 2 | ALA5113504 | organism-based format | Scientific Literature | |
| 18. | ALA5118592 | T | Cytotoxicity against human Huh-7 cells infected with Dengue virus serotype 2 assessed as reduction in cell viability by celltiter-blue assay | Homo sapiens | 2 | ALA5113504 | cell-based format | Scientific Literature | |
| 19. | ALA5118593 | A | Permeability of compound in PBS at pH 7.4 measured after 5 hrs by HPLC-UV based PAMPA assay | 9 | ALA5113504 | assay format | Scientific Literature | ||
| 20. | ALA5118594 | A | Chemical stability of the compound in SARS-CoV-2 main protease assay buffer assessed as SARS-CoV-2 main protease-mediated amide bond cleavage at 100 uM measured after 8 hrs by HPLC based UV-Vis analysis | Severe acute respiratory syndrome coronavirus 2 | 3 | ALA5113504 | single protein format | Scientific Literature |
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