| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5166981 | F | Anti-inflammatory activity against R848-stimulated mouse BMDC cells assessed as upregulation of IL-10 production at 5 uM treated for 24 hrs followed by R484 stimulation for 12 hrs by ELISA method (Rvb = 100 %) | Mus musculus | 96 | ALA5154907 | cell-based format | Scientific Literature | |
| 2. | ALA5166982 | F | Transcriptional activity of AP-1 in R848-stimulated mouse BMDC cells transfected with pAP1-luc assessed as ratio of AP-1 activity at 5 uM treated for 24 hrs followed by R484 stimulation for 4 hrs by luminescence based microplate reader analysis relative to control | Mus musculus | 34 | ALA5154907 | cell-based format | Scientific Literature | |
| 3. | ALA5166983 | T | Acute toxicity in 12 hr fasted ICR mouse assessed as toxic reaction at 2000 mg/kg, po measured daily for 7 days | Mus musculus | 1 | ALA5154907 | organism-based format | Scientific Literature | |
| 4. | ALA5166984 | A | Stability of compound in human liver microsome assessed as intrinsic clearance by HPLC analysis | Homo sapiens | 12 | ALA5154907 | microsome format | Scientific Literature | |
| 5. | ALA5166985 | B | Inhibition of recombinant full length His tagged human CDK8/Cyclin C expressed in Baculovirus incubated for 60 mins in presence of ATP by ADP-Glo kinase assay | Homo sapiens | 34 | ALA5154907 | assay format | Scientific Literature | |
| 6. | ALA5166986 | B | Binding affinity to CDK8 in human HCT-116 cells assessed as increase in thermal stability tolerance at 10 uM treated for 4 hrs followed by compound washout by CETSA assay | Homo sapiens | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 7. | ALA5166987 | B | Binding affinity to CDK8 in human HCT-116 cells assessed as thermal stability at 49 degC treated for 4 hrs followed by compound washout by CETSA assay | Homo sapiens | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 8. | ALA5166988 | B | Binding affinity to CDK8 in human HCT-116 cells assessed as reduction in protease-induced CDK8 degradation at 1 to 4 uM treated for 6 hrs followed by protease treatment for 30 mins by western blot analysis | Homo sapiens | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 9. | ALA5166989 | F | Anti-inflammatory activity against R848-stimulated mouse BMDC cells assessed as upregulation of IL-10 production by ELISA method | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 10. | ALA5166990 | F | Anti-inflammatory activity against R848 stimulated-mouse BMDC cells assessed as downregulation of IL-6 production by ELISA method | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 11. | ALA5166991 | B | Inhibition of CDK8 in IFN-gamma-stimulated mouse BMDC cells assessed as STAT-1 phosphorylation at Ser727 at 1 to 4 uM treated for 2 hrs followed by IFN-gamma stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 12. | ALA5166992 | B | Inhibition of JAK in IFN-gamma-stimulated mouse BMDC cells assessed as STAT-1 phosphorylation at Tyr701 at 1 to 4 uM treated for 2 hrs followed by IFN-gamma stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 13. | ALA5166993 | B | Inhibition of CDK8 in human HCT-116 cells assessed as STAT-1 phosphorylation at Ser727 at 1 to 4 uM treated for 24 hrs by western blot analysis | Homo sapiens | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 14. | ALA5166994 | B | Inhibition of CDK8 in human HCT-116 cells assessed as effect on CDK8 mediated STAT-1 phosphorylation at Tyr701 at 1 to 4 uM treated for 24 hrs by western blot analysis | Homo sapiens | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 15. | ALA5166995 | B | Inhibition of CDK8 in R848-stimulated mouse BMDC cells assessed as downregulation of c-Jun phosphorylation at Ser243 at 1 to 4 uM treated for 2 hrs followed by R848 stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 16. | ALA5166996 | B | Inhibition of CDK8 in R848-stimulated mouse BMDC cells assessed as effect on CRTC3 phosphorylation at Ser370 at 1 to 4 uM treated for 2 hrs followed by R848 stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 17. | ALA5166997 | B | Inhibition of TLR7 expression in R848-stimulated mouse BMDC cells at 1 to 4 uM treated for 2 hrs followed by R848 stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 18. | ALA5166998 | B | Inhibition of MyD88 expression in R848-stimulated mouse BMDC cells at 1 to 4 uM treated for 2 hrs followed by R848 stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 19. | ALA5166999 | B | Inhibition of NF-kappa-B phosphorylation in R848-stimulated mouse BMDC cells at 1 to 4 uM treated for 2 hrs followed by R848 stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature | |
| 20. | ALA5167000 | B | Inhibition of ERK phosphorylation in R848-stimulated mouse BMDC cells at 1 to 4 uM treated for 2 hrs followed by R848 stimulation for 12 hrs by western blot analysis | Mus musculus | 1 | ALA5154907 | cell-based format | Scientific Literature |
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