# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5153010 | F | Inhibition of polyamine uptake in chinese hamster CHO-K1 cells assessed as rescue of Ant44-induced toxicity by measuring cellular ATP content measured after 48 hrs in presence of Ant44 and aminoguanidine by ATPlite assay | Cricetulus griseus | 53 | ALA5150043 | cell-based format | Scientific Literature | |
2. | ALA5153011 | F | Inhibition of spermidine uptake chinese hamster CHO-K1 cells assessed as decrease in cell growth by measuring cellular ATP content measured after 48 hrs in presence of DFMO and spermidine by ATPlite assay | Cricetulus griseus | 53 | ALA5150043 | cell-based format | Scientific Literature | |
3. | ALA5153012 | T | Cytotoxicity against chinese hamster CHO-K1 cells assessed as decrease in cell viability measured after 48 hrs by ATPlite assay | Cricetulus griseus | 53 | ALA5150043 | cell-based format | Scientific Literature | |
4. | ALA5153013 | T | Cytotoxicity against human L3.6pl cells cells assessed as cell viability measured after 72 hrs by ATPlite assay | Homo sapiens | 20 | ALA5150043 | cell-based format | Scientific Literature | |
5. | ALA5153014 | T | Cytotoxicity against human L3.6pl cells cells assessed as inhibition of cell growth measured after 72 hrs by ATPlite assay | Homo sapiens | 20 | ALA5150043 | cell-based format | Scientific Literature | |
6. | ALA5153015 | F | Inhibition of polyamine uptake in human L3.6pl cells assessed as inhibition of Ant44-induced toxicity by measuring cellular ATP content at MTC measured after 72 hrs in presence of Ant44 by ATPlite assay | Homo sapiens | 20 | ALA5150043 | cell-based format | Scientific Literature | |
7. | ALA5153016 | F | Inhibition of spermidine uptake in human L3.6pl cells assessed as inhibition of cell growth by measuring cellular ATP content at MTC measured after 72 hrs in presence of DFMO and spermidine by ATPlite assay | Homo sapiens | 20 | ALA5150043 | cell-based format | Scientific Literature | |
8. | ALA5153017 | B | Inhibition of polyamine transport system in human L3.6pl cells assessed as reduction in [3H] spermidine uptake preincubated for 24 hrs followed by [3H]-spermidine addition and measured after 15 mins by scintillation counter method | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature | |
9. | ALA5153018 | B | Inhibition of polyamine transport system in human L3.6pl cells assessed as reduction in [3H] spermidine uptake preincubated for 72 hrs in presence of DFMO followed by [3H]-spermidine addition and measured after 15 mins by scintillation counter method | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature | |
10. | ALA5153019 | B | Inhibition of FUBP1/DNA FUSE region interaction in human L3.pl cells assessed as FUBP1 protein level at 20 to 60 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5150043 | cell-based format | Scientific Literature | |
11. | ALA5153020 | B | Inhibition of FUBP1/DNA FUSE region interaction in human L3.pl cells assessed as reduction in c-myc protein level at 20 to 60 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature | |
12. | ALA5153021 | B | Inhibition of FUBP1/DNA FUSE region interaction in human L3.pl cells assessed as increase in p21 protein level at 20 to 60 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature | |
13. | ALA5153022 | B | Inhibition of FUBP1/DNA FUSE region interaction in human L3.pl cells assessed as decrease in c-myc mRNA level at 20 to 60 uM incubated for 24 hrs by qRT-PCR analysis | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature | |
14. | ALA5153023 | B | Inhibition of FUBP1/DNA FUSE region interaction in human L3.pl cells assessed as increase in p21 mRNA level at 20 to 60 uM incubated for 24 hrs by qRT-PCR analysis | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature | |
15. | ALA5153024 | B | Inhibition of FUBP1/DNA FUSE region interaction in human L3.pl cells assessed as reduction in c-Myc FUSE DNA pull down at 60 uM incubated for 24 hrs by qRT-PCR analysis | Homo sapiens | 1 | ALA5150043 | cell-based format | Scientific Literature | |
16. | ALA5153025 | B | Binding affinity to recombinant GST tagged FUBP1 KH3-KH4 domain (unknown origin) assessed as decrease in FUBP1-FUSE DNA bound complex using 5'-IRDye700 tagged TGTATATTCCCTCGGG oligonucleotide as substrate at 30 to 60 uM measured for 30 mins by Gel shift assay relative to control | Homo sapiens | 1 | ALA5150043 | single protein format | Scientific Literature | |
17. | ALA5153026 | B | Binding affinity to recombinant GST tagged FUBP1 KH3-KH4 domain (unknown origin) assessed as decrease in FUBP1-FUSE DNA bound complex using 5'-IRDye700 tagged TATTCCCTGATTTTT oligonucleotide as substrate measured for 30 mins by Gel shift assay | Homo sapiens | 1 | ALA5150043 | single protein format | Scientific Literature | |
18. | ALA5153027 | F | Decrease in intracellular polyamine level in human L3.6pl cells assessed as total polyamine level at 60 uM incubated for 24 hrs by HPLC analysis | Homo sapiens | 1 | ALA5150043 | cell-based format | Scientific Literature | |
19. | ALA5153028 | F | Decrease in intracellular polyamine level in human L3.6pl cells assessed as putrescine level at 60 uM incubated for 24 hrs by HPLC analysis | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature | |
20. | ALA5153029 | F | Decrease in intracellular polyamine level in human L3.6pl cells assessed as spermidine level at 60 uM incubated for 24 hrs by HPLC analysis | Homo sapiens | 2 | ALA5150043 | cell-based format | Scientific Literature |