# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5142575 | B | Binding affinity to recombinant human spiked PKAC-alpha in human HeLa cells assessed as covalent compound-protein adduct formation by measuring multiple fluorescent band formation at 0.15 to 1 uM incubated for 1 hr by 5-TAMRA dye based Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
2. | ALA5142576 | A | Drug uptake in live human HeLa cells assessed as drug penetration by measuring increase in fluorescence intensity at 5 uM incubated for 2 hrs by PKAc antibody immunostaining based Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
3. | ALA5142577 | A | Drug uptake in human HeLa cell lysates assessed as drug penetration by measuring increase in fluorescence intensity at 1.5 uM incubated for 2 hrs by PKAc antibody immunostaining based Western blot analysis | Homo sapiens | 1 | subcellular format | Scientific Literature | ||
4. | ALA5142578 | B | Binding affinity to recombinant human PKAC-alpha in human HeLa cells assessed as increase in intensity of fluorescent labeled bands at 5 uM incubated for 2 hrs in presence of forskolin by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
5. | ALA5142579 | B | Binding affinity to recombinant human PKAC-alpha in human HeLa cells assessed as increase in intensity of fluorescent labeled bands at 1 uM incubated for 2 hrs in presence of forskolin by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |