| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5164910 | B | Inhibition of NLRC4 inflammasome activation in LPS/Salmonella-treated C57BL/6 mouse bone marrow-derived macrophages assessed as reduction in cleaved IL-1 beta level in culture supernatants preincubated for 3 hrs with LPS and for 30 mins with compound prior to Salmonella typhi addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA5164911 | B | Inhibition of NLRP3 inflammasome activation in LPS-stimulated mouse J774.A1 cells assessed as reduction in pyroptosis at 10 uM pretreated for 4 hrs with LPS and for 30 mins with compound prior to ATP addition and measured after 30 mins by PI/Hoechst33342 staining based analysis | Mus musculus | 33 | cell-based format | Scientific Literature | ||
| 3. | ALA5164912 | T | Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay | Homo sapiens | 33 | cell-based format | Scientific Literature | ||
| 4. | ALA5164913 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as reduction in IL-1beta secretion preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by ELISA | Mus musculus | 10 | cell-based format | Scientific Literature | ||
| 5. | ALA5164914 | A | Metabolic stability in human liver microsomes assessed as half life by LC-MS/MS analysis | Homo sapiens | 2 | microsome format | Scientific Literature | ||
| 6. | ALA5164915 | A | Metabolic stability in human liver microsomes assessed as intrinsic clearance by LC-MS/MS analysis | Homo sapiens | 2 | microsome format | Scientific Literature | ||
| 7. | ALA5164916 | A | Metabolic stability in human hepatocytes assessed as intrinsic clearance by LC-MS/MS analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 8. | ALA5164917 | B | Inhibition of NLRP3 inflammasome activation in LPS-stimulated mouse J774.A1 cells assessed as reduction in pyroptosis pretreated for 4 hrs with LPS and for 30 mins with compound prior to ATP addition and measured after 30 mins by PI/Hoechst33342 staining based analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA5164918 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as reduction in activated caspase 1(P20) level in culture supernatants preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA5164919 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as reduction in activated caspase 1(P20) level in cell lysates at 0.5 to 2 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | subcellular format | Scientific Literature | ||
| 11. | ALA5164920 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as pro-IL-1 beta level in cell lysates at 0.5 to 2 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | subcellular format | Scientific Literature | ||
| 12. | ALA5164921 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as pro-caspase-1 level in cell lysates at 0.5 to 2 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | subcellular format | Scientific Literature | ||
| 13. | ALA5164922 | F | Effect on NLRP3 expression in cell lysates of LPS/nigericin-stimulated C57BL/6 mouse bone marrow-derived macrophages at 0.5 to 2 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | subcellular format | Scientific Literature | ||
| 14. | ALA5164923 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as ASC level in cell lysates at 0.5 to 2 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | subcellular format | Scientific Literature | ||
| 15. | ALA5164924 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as NEK7 level in cell lysates at 0.5 to 2 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | subcellular format | Scientific Literature | ||
| 16. | ALA5164925 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as inhibition of ASC oligomerization preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA5164926 | F | Inhibition of LPS/nigericin-induced reactive oxygen species production in C57BL/6 mouse bone marrow-derived macrophages assessed as ROS level at 1 uM by DCFH-DA staining based flow cytometry (Rvb = 33.51%) | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5164927 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as reduction in NEK7-NLRP3 protein-protein interaction at 2 to 4 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by immunoprecipitation method | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA5164928 | B | Inhibition of NLRP3 inflammasome activation in LPS/nigericin-treated C57BL/6 mouse bone marrow-derived macrophages assessed as reduction in ASC-NLRP3 protein-protein interaction at 2 to 4 uM preincubated for 3 hrs with LPS and for 30 mins with compound prior to nigericin addition and measured after 30 mins by immunoprecipitation method | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA5164929 | B | Binding affinity to GFP-fused NLRP3 (unknown origin) expressed in HEK293T cells by MST assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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