Inhibition of OAT1 (unknown origin) expressed in HEK293 cells assessed as inhibition of 6-CFL uptake preincubated for 30 mins followed by incubation with 6-CFL and measured after 15 mins by fluorescence based analysis
Anti-hyperuricemic activity against xanthine and potassium oxonate-induced acute hyperuricemia Kunming mouse model assessed as serum uric acid concentration at 10 mg/kg, po measured after 3 hrs (Rvb = 846.22 uM)
Inhibition of URAT1 (unknown origin)-mediated 14C-uric acid uptake expressed in HEK293 cells using 14C-uric acid as substrate preincubated for 30 mins followed by substrate addition and measured after 15 mins by liquid scintillation counting analysis
Inhibition of GLUT9 (unknown origin) expressed in HEK293T cells assessed as inhibition of uric acid-induced current at 10 uM by whole cell patch clamp technique
Inhibition of URAT1 I481M mutant (unknown origin)-mediated 14C-uric acid uptake expressed in HEK293 cells using 14C-uric acid as substrate preincubated for 30 mins followed by substrate addition and measured after 15 mins by liquid scintillation counting analysis
Inhibition of ABCG2 (unknown origin) expressed in HEK293 cells membrane vesicles assessed as inhibition of ATP-induced 14C-uric acid uptake preincubated for 15 mins followed by incubation with ATP and substrate for 5 mins by liquid scintillation counting analysis
Anti-hyperuricemic activity against xanthine and potassium oxonate-induced acute hyperuricemia Kunming mouse model assessed as increase in urine uric acid concentration at 10 mg/kg, po measured for 24 hrs