#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5144467	B	Binding affinity to calf thymus DNA assessed as intercalation by measuring dissociation constant at 20 uM measured after 5 mins by UV-Vis spectrophotometric titration analysis	Bos taurus	7	ALA5143526	nucleic acid format	Scientific Literature
2.	ALA5144468	B	Binding affinity to calf thymus DNA assessed as ligand-induced thermal stabilization of DNA duplex by measuring change in melting temperature at 6.25 uM by thermal denaturation analysis	Bos taurus	7	ALA5143526	nucleic acid format	Scientific Literature
3.	ALA5144469	B	Displacement of ethidium bromide from calf thymus DNA incubated for 30 mins by fluorescence microplate reader assay	Bos taurus	7	ALA5143526	nucleic acid format	Scientific Literature
4.	ALA5144471	B	Inhibition of human recombinant MGMT expressed in Escherichia coli STAR BL21 pRare cells assessed as cleavage of fluorescent labelled substrate by Bsp 119I at 12.5 uM using O6MeG-bearing substrate (S) incubated for 5 mins by coupled enzymatic analysis relative to control	Homo sapiens	14	ALA5143526	cell-based format	Scientific Literature
5.	ALA5144472	B	Irreversible inhibition of human recombinant MGMT expressed in Escherichia coli STAR BL21 pRare cells assessed as cleavage of fluorescent labelled substrate by Bsp 119I at 12.5 uM using O6MeG-bearing substrate (S) preincubated for 30 mins followed by substrate addition and measured after 5 mins by coupled enzymatic analysis relative to control	Homo sapiens	8	ALA5143526	cell-based format	Scientific Literature
6.	ALA5144474	B	Competitive inhibition of human recombinant MGMT expressed in Escherichia coli STAR BL21 pRare cells assessed as cleavage of fluorescent labelled substrate by Bsp 119I at 12.5 uM using O6MeG-bearing substrate (S) incubated for 10 mins in presence of non-substrate CT-DNA followed by substrate addition and measured after 5 mins by coupled enzymatic analysis	Homo sapiens	7	ALA5143526	cell-based format	Scientific Literature
7.	ALA5144475	F	Antiproliferative activity against human T98G cells assessed as cell growth inhibition incubated for 96 hrs by CellTiter-Glo luminescent cell viability assay	Homo sapiens	14	ALA5143526	cell-based format	Scientific Literature
8.	ALA5144501	F	Additive antiproliferative activity against human T98G cells assessed as reduction in cell viability at 25 uM pretreated for 2 hrs followed by TMZ addition at 94 uM and measured after 94 hrs by CellTiter-Glo luminescent cell viability assay	Homo sapiens	4	ALA5143526	cell-based format	Scientific Literature
9.	ALA5144504	F	Additive antiproliferative activity against human T98G cells assessed as reduction in cell viability at GI50 pretreated for 2 hrs followed by TMZ addition at 188 uM and measured after 94 hrs by CellTiter-Glo luminescent cell viability assay	Homo sapiens	6	ALA5143526	cell-based format	Scientific Literature
10.	ALA5144510	F	Antagonistic antiproliferative activity against human T98G cells assessed as reduction in cell viability at GI50 pretreated for 2 hrs followed by TMZ addition at 375 uM and measured after 94 hrs by CellTiter-Glo luminescent cell viability assay	Homo sapiens	3	ALA5143526	cell-based format	Scientific Literature
11.	ALA5144511	P	Photostability of the compound in THF assessed as hypochromism at 10 uM by UV-visible spectrophotometry		6	ALA5143526	small-molecule physicochemical format	Scientific Literature
12.	ALA5144512	P	Photostability of the compound in MeCN assessed as hypochromism at 10 uM by UV-visible spectrophotometry		6	ALA5143526	small-molecule physicochemical format	Scientific Literature
13.	ALA5144513	P	Photostability of the compound in MeOH assessed as hypochromism at 10 uM by UV-visible spectrophotometry		6	ALA5143526	small-molecule physicochemical format	Scientific Literature
14.	ALA5144514	P	Photostability of the compound in in aqueous solution assessed as hypochromism at 10 uM by UV-visible spectrophotometry		6	ALA5143526	small-molecule physicochemical format	Scientific Literature
15.	ALA5144515	P	Thermal stability of compound in aqueous solution at 10 uM at 20 to 75 degreeC by UV-vis absorption spectroscopy		6	ALA5143526	small-molecule physicochemical format	Scientific Literature
16.	ALA5144516	P	Fluorescence property of compound in MeCN assessed as quantum yield		7	ALA5143526	small-molecule physicochemical format	Scientific Literature
17.	ALA5144517	P	Fluorescence property of compound in THF assessed as quantum yield		7	ALA5143526	small-molecule physicochemical format	Scientific Literature
18.	ALA5144518	P	Fluorescence property of compound in MeOH assessed as quantum yield		7	ALA5143526	small-molecule physicochemical format	Scientific Literature
19.	ALA5144519	P	Fluorescence property of compound in aqueous solution assessed as quantum yield		7	ALA5143526	small-molecule physicochemical format	Scientific Literature