| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5135014 | Binding | Antagonist activity at PAC-1 receptor (unknown origin) expressed in CHO cells assessed as inhibition of PACAP-induced CREB phosphorylation at 1 nM pretreated for 30 mins followed by PACAP stimulation for 30 mins by western blot analysis | Homo sapiens | 20 | ALA5131488 | cell-based format | Scientific Literature | |
| 2. | ALA5135015 | Binding | Agonist activity at PAC-1 receptor (unknown origin) expressed in CHO cells assessed as increase in intracellular cAMP level at 0.001 to 100 nM treated for 30 mins by cAMP-Glo assay | Homo sapiens | 1 | ALA5131488 | cell-based format | Scientific Literature | |
| 3. | ALA5135016 | Functional | Antagonist activity at VPAC-2 receptor (unknown origin) expressed in CHO cells assessed as inhibition of PACAP-induced increase in intracellular cAMP level at 0.001 to 100 nM pretreated for 30 mins followed by PACAP stimulation measured after 1 hr by cAMP-Glo assay | Homo sapiens | 1 | ALA5131488 | cell-based format | Scientific Literature | |
| 4. | ALA5135017 | Functional | Inhibition of PACAP-induced aversive behaviour in it dosed ddY mouse administered as single dose and measured for 30 mins | Mus musculus | 3 | ALA5131488 | organism-based format | Scientific Literature | |
| 5. | ALA5135018 | Binding | Antagonist activity at PAC-1 receptor (unknown origin) expressed in CHO cells assessed as inhibition of PACAP-induced increase in intracellular cAMP level pretreated for 30 mins followed by PACAP stimulation measured after 1 hr by cAMP-Glo assay | Homo sapiens | 2 | ALA5131488 | cell-based format | Scientific Literature | |
| 6. | ALA5135019 | Functional | Antagonist activity at VPAC-1 receptor (unknown origin) expressed in CHO cells assessed as inhibition of PACAP-induced increase in intracellular cAMP level at 0.001 to 100 nM pretreated for 30 mins followed by PACAP stimulation measured after 1 hr by cAMP-Glo assay | Homo sapiens | 1 | ALA5131488 | cell-based format | Scientific Literature | |
| 7. | ALA5135020 | Functional | Agonist activity at VPAC-1 receptor (unknown origin) expressed in CHO cells assessed as increase in intracellular cAMP level at 0.001 to 100 nM treated for 30 mins by cAMP-Glo assay | Homo sapiens | 1 | ALA5131488 | cell-based format | Scientific Literature | |
| 8. | ALA5135021 | Functional | Agonist activity at VPAC-2 receptor (unknown origin) expressed in CHO cells assessed as increase in intracellular cAMP level at 0.001 to 100 nM treated for 30 mins by cAMP-Glo assay | Homo sapiens | 1 | ALA5131488 | cell-based format | Scientific Literature | |
| 9. | ALA5135022 | Functional | Antiallodynic activity in ddY mouse assessed as inhibition of PACAP-evoked mechanical allodynia at 10 to 100 pmol administered via IT as single dose and measured after 14 days | Mus musculus | 3 | ALA5131488 | organism-based format | Scientific Literature | |
| 10. | ALA5135023 | Functional | Antiallodynic activity in ddY mouse assessed as inhibition of spinal nerve ligation-evoked mechanical allodynia at 10 pmol administered via IT as single dose and measured after 3 hrs | Mus musculus | 2 | ALA5131488 | organism-based format | Scientific Literature | |
| 11. | ALA5135024 | Functional | Antiallodynic activity in ddY mouse assessed as inhibition of spinal nerve ligation-evoked mechanical allodynia at 10 pmol administered via IT as single dose and measured after 2 to 4 hrs | Mus musculus | 2 | ALA5131488 | organism-based format | Scientific Literature | |
| 12. | ALA5135025 | Physicochemical | Aqueous solubility of the compound in phosphate buffered saline at 100 uM at pH 7.4 by HPLC analysis | 3 | ALA5131488 | small-molecule physicochemical format | Scientific Literature | ||
| 13. | ALA5135026 | ADME | Permeability of the compound across apical to basolateral side in human Caco-2 cells at 25 uM incubated for 2 hrs by LC/MS/MS analysis | Homo sapiens | 3 | ALA5131488 | cell-based format | Scientific Literature | |
| 14. | ALA5135027 | ADME | Metabolic stability in human liver microsomes assessed as parent compound remaining in presence of NADPH at 0.5 uM measured after 10 mins by LC-MS/MS analysis | Homo sapiens | 3 | ALA5131488 | microsome format | Scientific Literature | |
| 15. | ALA5135028 | ADME | Metabolic stability in mouse liver microsomes assessed as parent compound remaining in presence of NADPH at 0.5 uM measured after 10 mins by LC-MS/MS analysis | Mus musculus | 3 | ALA5131488 | microsome format | Scientific Literature | |
| 16. | ALA5135029 | ADME | Metabolic stability in human liver microsomes assessed as parent compound remaining in presence of NADPH at 0.5 uM measured after 60 mins by LC-MS/MS analysis | Homo sapiens | 3 | ALA5131488 | microsome format | Scientific Literature | |
| 17. | ALA5135030 | ADME | Metabolic stability in mouse liver microsomes assessed as parent compound remaining in presence of NADPH at 0.5 uM measured after 60 mins by LC-MS/MS analysis | Mus musculus | 3 | ALA5131488 | microsome format | Scientific Literature | |
| 18. | ALA5135031 | ADME | Plasma protein binding in human measured after 5 hrs by LC-MS/MS analysis | Homo sapiens | 3 | ALA5131488 | cell-free format | Scientific Literature | |
| 19. | ALA5135032 | ADME | Plasma protein binding in mouse measured after 5 hrs by LC-MS/MS analysis | Mus musculus | 3 | ALA5131488 | cell-free format | Scientific Literature | |
| 20. | ALA5135033 | ADME | Cmax in C57BL/6J mouse at 30 mg/kg, ip measured up to 24 hrs by LC-MS/MS analysis | Mus musculus | 3 | ALA5131488 | organism-based format | Scientific Literature |
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