| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5115882 | B | Inhibition of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells using FAM-labeled ssRNA (5'-CUCGAUACG(m^6A) UCCGGUCAAA-3') as substrate incubated for 60 mins by fluorescence polarization assay | Homo sapiens | 51 | cell-based format | Scientific Literature | ||
| 2. | ALA5115883 | B | Stabilization of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as melting temperature measured at 25 to 95 degree C by differential scanning fluorimetry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA5115884 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 100 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA5115885 | B | Inhibition of of human recombinant ALKBH5 (66 to 292 residues) mediated demethylation of m6A substrate expressed in Escherichia coli BL21 (DE3) cells incubated for 2 hrs by dot blot assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA5115886 | B | Inhibition of FTO (unknown origin) mediated demethylation of m6A substrate at 0.1 to 10 uM incubated for 2 hrs by dot blot assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 6. | ALA5115887 | B | Binding affinity to FTO (unknown origin) demethylation activity assessed as thermal stability by measuring change in melting temperature at 100 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA5115888 | B | Binding affinity to ALKHB1 (unknown origin) demethylation activity assessed as thermal stability by measuring change in melting temperature at 100 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA5115889 | B | Binding affinity to ALKHB2 (unknown origin) demethylation activity assessed as thermal stability by measuring change in melting temperature at 100 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 9. | ALA5115890 | B | Binding affinity to human recombinant ALKBH5 in human HepG2 cells assessed as thermal stability at 100 uM measured for 3 hrs by cellular thermal shift assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA5115891 | B | Inhibition of human recombinant ALKBH5 in human HepG2 cells assessed as increase in m6A level incubated for 72 hrs by dot blot assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA5115892 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 50 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA5115893 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 25 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA5115894 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 6.25 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA5115895 | B | Thermodynamic stability of human recombinant ALKBH5 (66 to 292 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 1.56 uM by differential scanning fluorimetry assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5115896 | B | Inhibition of ALKHB1 (unknown origin) mediated demethylation of m6A assessed as enzyme substrate binding by fluorescence polarisation assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA5115897 | B | Inhibition of FTO (unknown origin) mediated demethylation of m6A assessed as disruption of enzyme substrate binding by fluorescence polarisation assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA5115898 | B | Inhibition of ALKHB5 (unknown origin) mediated demethylation of m6A assessed as enzyme substrate binding by fluorescence polarisation assay | Homo sapiens | 1 | single protein format | Scientific Literature |
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