# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5130301 | F | Antiproliferative activity against human MCF7-DOX cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
2. | ALA5130302 | F | Induction of apoptosis in human A549/DDP cells assessed as early apoptotic cells incubated for 48 hrs by Annexin V-FITC/Propidium iodide staining based flowcytometry analysis (Rvb = 0 %) | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
3. | ALA5130303 | B | Inhibition of human recombinant AKR1C1 transfected in Escherichia coli BL21 (DE3) pLysS competent cells assessed as inhibition of NADP+ dependent oxidation of S-tetralol using S-tetralol as substrate incubated for 10 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA5126618 | assay format | Scientific Literature | |
4. | ALA5130304 | B | Inhibition of human recombinant AKR1C2 transfected in Escherichia coli BL21 (DE3) pLysS competent cells assessed as inhibition of NADP+ dependent oxidation of S-tetralol using S-tetralol as substrate incubated for 10 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA5126618 | assay format | Scientific Literature | |
5. | ALA5130305 | B | Inhibition of human recombinant AKR1C3 transfected in Escherichia coli BL21 (DE3) pLysS competent cells assessed as inhibition of NADP+ dependent oxidation of S-tetralol using S-tetralol as substrate incubated for 10 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA5126618 | assay format | Scientific Literature | |
6. | ALA5130306 | B | Inhibition of human recombinant AKR1C4 transfected in Escherichia coli BL21 (DE3) pLysS competent cells assessed as inhibition of NADP+ dependent oxidation of S-tetralol using S-tetralol as substrate incubated for 10 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA5126618 | assay format | Scientific Literature | |
7. | ALA5130307 | B | Selectivity index, ratio of IC50 for inhibition of human recombinant AKR1C1 transfected in Escherichia coli BL21 (DE3) pLysS competent cells to IC50 for inhibition of human recombinant AKR1C3 transfected in Escherichia coli BL21 (DE3) pLysS competent cells | Homo sapiens | 5 | ALA5126618 | assay format | Scientific Literature | |
8. | ALA5130308 | B | Selectivity index, ratio of IC50 for inhibition of human recombinant AKR1C2 transfected in Escherichia coli BL21 (DE3) pLysS competent cells to IC50 for inhibition of human recombinant AKR1C3 transfected in Escherichia coli BL21 (DE3) pLysS competent cells | Homo sapiens | 5 | ALA5126618 | assay format | Scientific Literature | |
9. | ALA5130309 | B | Selectivity index, ratio of IC50 for inhibition of human recombinant AKR1C4 transfected in Escherichia coli BL21 (DE3) pLysS competent cells to IC50 for inhibition of human recombinant AKR1C3 transfected in Escherichia coli BL21 (DE3) pLysS competent cells | Homo sapiens | 5 | ALA5126618 | assay format | Scientific Literature | |
10. | ALA5130310 | F | Antiproliferative activity against human A549/DDP cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
11. | ALA5130315 | F | Potentiation of doxorubicin induced antiproliferative activity against human MCF7-DOX cells assessed as reduction in cell viability at 10 uM incubated for 48 hrs in presence of 25 uM doxorubicin by MTT assay relative to control | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
12. | ALA5130317 | F | Synergistic antiproliferative activity against human MCF7-DOX cells assessed as reduction in cell viability at 50 to 200 uM incubated for 48 hrs in presence of doxorubicin by MTT assay | Homo sapiens | 5 | ALA5126618 | cell-based format | Scientific Literature | |
13. | ALA5130318 | F | Induction of apoptosis in human A549/DDP cells assessed as late apoptotic cells incubated for 48 hrs by Annexin V-FITC/Propidium iodide staining based flowcytometry analysis (Rvb = 0.01 %) | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
14. | ALA5130319 | F | Induction of apoptosis in human A549/DDP cells assessed as viable cells incubated for 48 hrs in presence of cisplatin by Annexin V-FITC/Propidium iodide staining based flowcytometry analysis (Rvb = 99.99 %) | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
15. | ALA5130320 | F | Reversal of cisplatin resistance in human A549/DDP cells assessed as inhibition of cell proliferation at low concentrations incubated for 48 hrs by MTT assay | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
16. | ALA5130322 | F | Synergistic antiproliferative activity against human A549/DDP cells assessed as combination index at 50 to 200 uM incubated for 48 hrs in presence of cisplatin by MTT assay | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
17. | ALA5130327 | F | Induction of apoptosis in human MCF7-DOX cells assessed as reduction in number of viable cells incubated for 48 hrs in presence of doxorubicin by Hoechst 33342 staining based fluorescence microscopic analysis | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
18. | ALA5130328 | F | Induction of apoptosis in human MCF7-DOX cells assessed as increase in chromatin condensation incubated for 48 hrs in presence of doxorubicin by Hoechst 33342 staining based fluorescence microscopic analysis | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
19. | ALA5130329 | F | Induction of apoptosis in human MCF7-DOX cells assessed as increase in nuclear fragmentation incubated for 48 hrs in presence of doxorubicin by Hoechst 33342 staining based fluorescence microscopic analysis | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature | |
20. | ALA5130330 | F | Induction of apoptosis in human A549/DDP cells assessed as viable cells incubated for 48 hrs by Annexin V-FITC/Propidium iodide staining based flowcytometry analysis (Rvb = 99.99 %) | Homo sapiens | 1 | ALA5126618 | cell-based format | Scientific Literature |