# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5118572 | B | Inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) using 2-Abz-Ser-AlaVal-Leu-Gln-Ser-Gly-Tyr(3-NO2)-Arg-OH as substrate preincubated for 15 mins followed by substrate addition and measured after 15 mins by FRET assay | Severe acute respiratory syndrome coronavirus 2 | 37 | ALA5113504 | assay format | Scientific Literature | |
2. | ALA5118573 | B | Inhibition of C-terminal His tagged SARS CoV-2 main protease transfected in Escherichia coli BL21 (DE3) using 2-Abz-Ser-AlaVal-Leu-Gln-Ser-Gly-Tyr(3-NO2)-Arg-OH as substrate at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by FRET assay relative to control | Severe acute respiratory syndrome coronavirus 2 | 37 | ALA5113504 | assay format | Scientific Literature | |
3. | ALA5118574 | B | Inhibition of full length DENV2 NS2B-NS3 protease in human HeLa cells expressing luciferase reporter gene incubated for 24 hrs by luminescence analysis | dengue virus type 2 | 37 | ALA5113504 | cell-based format | Scientific Literature | |
4. | ALA5118575 | B | Inhibition of full length DENV2 NS2B-NS3 protease in human HeLa cells expressing luciferase reporter gene at 12.5 uM incubated for 24 hrs by luminescence analysis relative to control | dengue virus type 2 | 37 | ALA5113504 | cell-based format | Scientific Literature | |
5. | ALA5118576 | T | Cytotoxicity against human HeLa cells expressing luciferase-based DENV-2 protease reporter system assessed as reduction in cell viability by celltiter-blue assay | Homo sapiens | 37 | ALA5113504 | cell-based format | Scientific Literature | |
6. | ALA5118588 | B | Inhibition of trypsin (unknown origin) using Boc-Val-Pro-Arg-AMC as substrate assessed as enzymatic cleavage at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by continuous fluorimetric assay | Homo sapiens | 33 | ALA5113504 | protein format | Scientific Literature | |
7. | ALA5118589 | B | Inhibition of thrombin (unknown origin) using Boc-Val-Pro-Arg-AMC as substrate assessed as enzymatic cleavage at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by continuous fluorimetric assay | Homo sapiens | 34 | ALA5113504 | single protein format | Scientific Literature | |
8. | ALA5118596 | B | Inhibition of full length DENV2 NS2B-NS3 protease using 2-Abz-Nle-Lys-Arg-Arg-Ser-Tyr(3-NO2)-NH2 as substrate at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by FRET assay | dengue virus type 2 | 34 | ALA5113504 | single protein format | Scientific Literature | |
9. | ALA5118597 | B | Inhibition of WNV NS2B-NS3 protease using 2-Abz-Gly-Lys-Lys-Arg-Gly-Tyr(3-NO2)-Ala-Lys-NH2 as substrate at 50 uM preincubated for 15 mins followed by substrate addition and measured after 15 mins by FRET assay | West Nile virus | 34 | ALA5113504 | assay format | Scientific Literature |