#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5156922	B	Activation of Nrf2 transcriptional activity in HEK293T cells transfected with TK-Renilla reporter vector and cotransfected with ARE-LUC vector assessed as concentration required to double luciferase activity measured after 16 hrs by dual-luciferase reporter gene assay	Homo sapiens	3		cell-based format	Scientific Literature
2.	ALA5156923	B	Activation of Nrf2 transcriptional activity in HEK293T cells transfected with TK-Renilla reporter vector and cotransfected with ARE-LUC vector measured after 16 hrs by dual-luciferase reporter gene assay	Homo sapiens	3		cell-based format	Scientific Literature
3.	ALA5156924	B	Activation of Nrf2 transcriptional activity in KEAP1+/+ mouse embryonic fibroblast cells assessed as increase in HMOX1 mRNA levels at 20 uM by RT-qPCR analysis	Mus musculus	1		cell-based format	Scientific Literature
4.	ALA5156925	B	Inhibition of Nrf2 (unknown origin)/KEAP1 (unknown origin) protein-protein interaction at 100 uM by Fluorescence Polarization assay	Homo sapiens	1		protein format	Scientific Literature
5.	ALA5156926	B	Activation of Nrf2 transcriptional activity in KEAP1-/- mouse embryonic fibroblast cells assessed as increase in HMOX1 mRNA levels at 20 uM measured upto 24 hrs by RT-qPCR analysis	Mus musculus	1		cell-based format	Scientific Literature
6.	ALA5156927	B	Inhibition of ROCK2 (unknown origin) using KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK-peptide as substrate incubated for 30 mins in presence of [gamma-33P]-ATP	Homo sapiens	9		single protein format	Scientific Literature
7.	ALA5156928	F	Antioxidant activity assessed as DPPH radical scavenging activity incubated for 30 mins by spectrophotometric analysis		10		assay format	Scientific Literature
8.	ALA5156929	A	Stability in pooled human liver microsomes assessed as half life at 8 uM in presence of NADPH measured upto 60 mins by HPLC-MS analysis	Homo sapiens	2		microsome format	Scientific Literature
9.	ALA5156930	A	Stability in pooled human liver microsomes assessed as intrinsic clearance at 8 uM in presence of NADPH measured upto 60 mins by HPLC-MS analysis	Homo sapiens	2		microsome format	Scientific Literature
10.	ALA5156931	A	Binding affinity to human serum albumin assessed as Stern-Volmer quenching constant by Stern-Volmer plot analysis	Homo sapiens	1		single protein format	Scientific Literature
11.	ALA5156932	A	Binding affinity to human serum albumin assessed as association constant by Scatchard equation	Homo sapiens	1		single protein format	Scientific Literature
12.	ALA5156933	A	Binding affinity to human serum albumin assessed as decrease in fluorescence intensity at 0.2 to 20 uM incubated for 5 mins by fluorescence analysis	Homo sapiens	1		single protein format	Scientific Literature
13.	ALA5156934	T	Cytotoxicity against HEK293T cells assessed as reduction in cell viability at 20 uM measured after 16 hrs by MTT assay	Homo sapiens	10		cell-based format	Scientific Literature
14.	ALA5156935	T	Cytotoxicity against HEK293T cells assessed as reduction in cell viability at 60 uM measured after 16 hrs by MTT assay	Homo sapiens	10		cell-based format	Scientific Literature
15.	ALA5156936	B	Selectivity ratio of CD for Nrf2 activation in HEK293T cells to IC50 for inhibition of ROCK2 (unknown origin)	Homo sapiens	3		cell-based format	Scientific Literature
16.	ALA5156937	B	Activation of Nrf2 transcriptional activity in human SH-SY5Y cells assessed as induction of accumulation of Nrf2 in nucleus at 20 uM incubated for 30 to 240 mins by Immunoblotting analysis	Homo sapiens	1		cell-based format	Scientific Literature
17.	ALA5156938	B	Activation of Nrf2 transcriptional activity in human SH-SY5Y cells assessed as induction of accumulation of Nrf2 in cytosol at 20 uM incubated for 30 to 240 mins by Immunoblotting analysis	Homo sapiens	1		subcellular format	Scientific Literature
18.	ALA5156939	B	Activation of Nrf2 transcriptional activity in human SH-SY5Y cells assessed as increase in HMOX1 mRNA expression at 20 uM by RT-qPCR analysis	Homo sapiens	1		cell-based format	Scientific Literature
19.	ALA5156940	B	Activation of Nrf2 transcriptional activity in human SH-SY5Y cells assessed as increase in NQO1 mRNA expression at 20 uM by RT-qPCR analysis	Homo sapiens	1		cell-based format	Scientific Literature
20.	ALA5156941	B	Activation of Nrf2 transcriptional activity in human SH-SY5Y cells assessed as increase in HO-1 protein levels at 20 uM incubated for 24 hrs by immunoblot analysis	Homo sapiens	1		cell-based format	Scientific Literature