| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5123584 | B | Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 5 mins followed by substrate addition and measured after 1 min in the presence of DCHBS by horseradish peroxidase coupled colorimetric method relative to control | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 2. | ALA5123585 | B | Inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 5 mins followed by substrate addition and measured after 1 min in the presence of DCHBS by horseradish peroxidase coupled colorimetric method relative to control | Mus musculus | 6 | cell-based format | Scientific Literature | ||
| 3. | ALA5123588 | B | Inhibition of N-terminal His-tagged mouse recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay relative to control | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 4. | ALA5123589 | B | Inhibition of N-terminal His-tagged human recombinant glycolate oxidase expressed in Escherichia coli BL21 (DE3) cells using glycolate as substrate at 20 uM preincubated for 10 mins followed by substrate addition and measured after 10 mins by Amplex red and horseradish peroxidase based fluorescence assay relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 5. | ALA5123590 | B | Inhibition of human recombinant LDHA using pyruvate as substrate preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay | Homo sapiens | 10 | single protein format | Scientific Literature | ||
| 6. | ALA5123592 | B | Inhibition of human recombinant LDHB using pyruvate as substrate at 10 uM preincubated for 10 mins followed by substrate addition and measured every 60 sec for 10 mins in the presence of NADH by fluorescence assay relative to control | Homo sapiens | 1 | single protein format | Scientific Literature |
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