#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5160158	Binding	Protac activity at VHL/SOS2 in human NCI-H358 cells assessed as SOS2 protein degradation at 9.8 to 2500 nM incubated for 24 hr by Western blot analysis	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
2.	ALA5160159	Binding	Protac activity at SOS1/KRAS E3 ligase in human NCI-H358 cells assessed as KRAS protein level at 2.5 uM measured after 24 hr by qRT PCR analysis	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
3.	ALA5160160	Binding	Protac activity at VHL/SOS1 in human NCI-H358 cells assessed as SOS1 protein degradation at 0.1 uM incubated for 24 hr by Western blot analysis relative to control	Homo sapiens	16	ALA5154769	cell-based format	Scientific Literature
4.	ALA5160161	Binding	Protac activity at VHL/SOS1 in human NCI-H358 cells assessed as SOS1 protein degradation at 1 uM incubated for 24 hr by Western blot analysis relative to control	Homo sapiens	16	ALA5154769	cell-based format	Scientific Literature
5.	ALA5160162	Binding	Protac activity at VHL/SOS1 in human NCI-H358 cells assessed as SOS1 protein degradation incubated for 24 hr by Western blot analysis	Homo sapiens	4	ALA5154769	cell-based format	Scientific Literature
6.	ALA5160163	Binding	Protac activity at VHL/SOS1 in human NCI-H358 cells assessed as SOS1 protein degradation at 1 uM and measured after 4 to 24 hr by western blot analysis	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
7.	ALA5160164	Binding	Protac activity at VHL/SOS1 in human ASPC1 cells assessed as SOS1 protein degradation at 1 uM incubated measured after 16 hr by western blot analysis	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
8.	ALA5160167	Binding	Protac activity at VHL/SOS1 in human NCI-H358 cells assessed as SOS1 protein degradation pretreated with VHL ligand and MG132 at 1 uM followed by compound addition and measured after 24 hr by western blot analysis	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
9.	ALA5160168	Binding	Protac activity at VHL/SOS1 in human ASPC1 cells assessed as SOS1 protein degradation pretreated with VHL ligand and MG132 at 1 uM followed by compound addition and measured after 24 hr by western blot analysis	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
10.	ALA5160169	Functional	In vivo inhibition of phosphorylated ERK level in BALB/c nude mouse xenografted with human NCI-H358 cells at 20 to 50 mg/kg, IT incubated for 21 days by immunohistochemistry analysis	Mus musculus	1	ALA5154769	organism-based format	Scientific Literature
11.	ALA5160170	Binding	Binding affinity to SOS1/VCB E3 ligase (unknown origin) assessed as ternary complex formation by measuring hook effect incubated for 1 hr by HTRF assay	Homo sapiens	1	ALA5154769	assay format	Scientific Literature
12.	ALA5160171	Binding	Binding affinity to VCB E3 ligase (unknown origin) assessed as binary equilibrium dissociation constant by surface plasmon resonance analysis	Homo sapiens	1	ALA5154769	protein complex format	Scientific Literature
13.	ALA5160172	Binding	Binding affinity to SOS1/VCB E3 ligase (unknown origin) assessed as ternary dissociation constant by surface plasmon resonance analysis	Homo sapiens	1	ALA5154769	assay format	Scientific Literature
14.	ALA5160173	Binding	Co-operativity factor, Ratio of Kd for Binding affinity to VCB E3 ligase (unknown origin) assessed as binary equilibrium dissociation constant to Kd for binding affinity to SOS1/VCB E3 ligase (unknown origin) assessed as ternary dissociation constant	Homo sapiens	1	ALA5154769	assay format	Scientific Literature
15.	ALA5160174	Binding	Protac activity at VHL/SOS2 in human NCI-H358 cells assessed as SOS2 mRNA level at 1 uM incubated for 24 to 72 hr by qRT PCR analysis	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
16.	ALA5160175	Functional	Antiproliferative activity against human NCI-H358 cells harboring KRAS G12C mutant assessed as growth inhibition incubated for 7 days by CellTiter-Glo 3D cell viability assay	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
17.	ALA5160176	Functional	Antiproliferative activity against human MIA PaCa-2 cells harboring KRAS G12C mutant assessed as cell growth inhibition incubated for 7 days by CellTiter-Glo 3D cell viability assay	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
18.	ALA5160177	Functional	Antiproliferative activity against human ASPC1 cells harboring KRAS G12D mutant assessed as cell growth inhibition incubated for 7 days by CellTiter-Glo 3D cell viability assay	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
19.	ALA5160178	Functional	Antiproliferative activity against human SK-LU-1 cells harboring KRAS G12D mutant assessed as cell growth inhibition incubated for 7 days by CellTiter-Glo 3D cell viability assay	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature
20.	ALA5160179	Functional	Antiproliferative activity against human SW620 cells harboring KRAS G12V mutant assessed as cell growth inhibition incubated for 7 days by CellTiter-Glo 3D cell viability assay	Homo sapiens	1	ALA5154769	cell-based format	Scientific Literature