# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5148453 | B | Inhibition of recombinant Trypanosoma brucei PTR1 expressed in Escherichia coli BL21 (DE3) cells using H2B as substrate in presence of cytochrome c and NADPH by envision multilabel reader | Trypanosoma brucei | 34 | ALA5143713 | cell-based format | Scientific Literature | |
2. | ALA5148454 | B | Inhibition of recombinant Leishmania major PTR1 expressed in Escherichia coli BL21 (DE3) cells using H2B as substrate in presence of cytochrome c and NADPH by envision multilabel reader | Leishmania major | 34 | ALA5143713 | cell-based format | Scientific Literature | |
3. | ALA5148455 | B | Inhibition of recombinant Trypanosoma brucei DHFR-TS expressed in Escherichia coli BL21 (DE3) cells using dihydrofolate as substrate in presence of NADPH by spectrophotometric analysis | Trypanosoma brucei | 34 | ALA5143713 | cell-based format | Scientific Literature | |
4. | ALA5148456 | B | Inhibition of recombinant Leishmania major DHFR-TS expressed in Escherichia coli BL21 (DE3) cells using dihydrofolate as substrate in presence of NADPH by spectrophotometric analysis | Leishmania major | 34 | ALA5143713 | cell-based format | Scientific Literature | |
5. | ALA5148457 | T | Inhibition of recombinant human DHFR expressed in Escherichia coli BL21 (DE3) cells using dihydrofolate as substrate in presence of NADPH by spectrophotometric analysis | Homo sapiens | 34 | ALA5143713 | cell-based format | Scientific Literature | |
6. | ALA5148458 | T | Selectivity index, ratio of IC50 for human DHFR to IC50 for Trypanosoma brucei PTR1 | 34 | ALA5143713 | assay format | Scientific Literature | ||
7. | ALA5148459 | T | Selectivity index, ratio of IC50 for human DHFR to IC50 for Leishmania major PTR1 | 33 | ALA5143713 | assay format | Scientific Literature | ||
8. | ALA5148460 | T | Selectivity index, ratio of IC50 for human DHFR to IC50 for Trypanosoma brucei DHFR | 34 | ALA5143713 | assay format | Scientific Literature | ||
9. | ALA5148461 | T | Selectivity index, ratio of IC50 for human DHFR to IC50 for Leishmania major DHFR | 34 | ALA5143713 | assay format | Scientific Literature | ||
10. | ALA5148466 | T | Inhibition of hERG potassium channel at 10 uM by fluorescence polarization assay relative to control | Homo sapiens | 33 | ALA5143713 | assay format | Scientific Literature | |
11. | ALA5148467 | A | Inhibition of CYP1A2 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control | Homo sapiens | 33 | ALA5143713 | cell-based format | Scientific Literature | |
12. | ALA5148468 | A | Inhibition of CYP2C9 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control | Homo sapiens | 33 | ALA5143713 | cell-based format | Scientific Literature | |
13. | ALA5148469 | A | Inhibition of CYP2C19 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control | Homo sapiens | 33 | ALA5143713 | cell-based format | Scientific Literature | |
14. | ALA5148470 | A | Inhibition of CYP2D6 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control | Homo sapiens | 33 | ALA5143713 | cell-based format | Scientific Literature | |
15. | ALA5148471 | A | Inhibition of CYP3A4 (unknown origin) expressed in baculovirus-infected insect cells at 10 uM using CYP/Luciferin as substrate incubated for 30 mins in presence of NADPH by luminescence based analysis relative to control | Homo sapiens | 33 | ALA5143713 | cell-based format | Scientific Literature | |
16. | ALA5148472 | T | Mitochondrial toxicity in human 786-0 cells at 10 uM incubated with compound for 6 hrs followed by incubation with MitoTracker Red chloromethyl-X-rosamine dye for 45 mins by imaging analysis relative to control | Homo sapiens | 33 | ALA5143713 | cell-based format | Scientific Literature | |
17. | ALA5148473 | T | Cytotoxicity against human A549 cells at 10 uM measured after 48 hrs by Celltiter-Glo assay relative to control | Homo sapiens | 33 | ALA5143713 | cell-based format | Scientific Literature |