# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5129607 | B | Displacement of carboxyfluorescein labelled NFATc2 derived PRIEIT peptide from human CNAalpha (2 to 347 residues) at 100 uM preincubated for 15 mins followed by CF-PRIEIT addition and measured after 15 mins by fluorescence polarization assay | Homo sapiens | 11 | single protein format | Scientific Literature | ||
2. | ALA5129609 | B | Inhibition of human recombinant CNA (2 to 347 residues) phosphatase activity at 50 uM using p-nitrophenyl phosphate as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins | Homo sapiens | 11 | single protein format | Scientific Literature | ||
3. | ALA5129610 | B | Inhibition of NFATC activity in HEK293T cells at 5 to 50 uM preincubated for 2 hrs followed by ionomycin/phorbol 12-myristate 13-acetate stimulation and measured after 4 hrs by luciferase reporter assay | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
4. | ALA5129611 | B | Inhibition of NFATC activity in HEK293T cells at 50 uM preincubated for 2 hrs followed by ionomycin/phorbol 12-myristate 13-acetate stimulation and measured after 4 hrs by luciferase reporter assay | Homo sapiens | 10 | cell-based format | Scientific Literature |