| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5156182 | Binding | Inhibition of Cy3-labeled mouse preE-let-7f-1 miRNA binding to human LIN28A (16 to 187 residues) expressed in Escherichia coli BL21 (DE3) at 75 uM preincubated for 2 hrs followed by miRNA addition and measured after 15 mins by electrophoretic mobility shift assay relative to control | 17 | ALA5154666 | assay format | Scientific Literature | ||
| 2. | ALA5156183 | Binding | Inhibition of Cy3-labeled mouse preE-let-7f-1 miRNA binding to human LIN28A (16 to 187 residues) expressed in Escherichia coli BL21 (DE3) preincubated for 2 hrs followed by miRNA addition and measured after 15 mins by electrophoretic mobility shift assay | 3 | ALA5154666 | assay format | Scientific Literature | ||
| 3. | ALA5156184 | Binding | Inhibition of FAM-labeled mouse preE-let-7f-1 miRNA binding to human LIN28A (16 to 187 residues) expressed in Escherichia coli BL21 (DE3) preincubated for 30 mins followed by miRNA addition by fluorescence polarization assay | 2 | ALA5154666 | assay format | Scientific Literature | ||
| 4. | ALA5156185 | Functional | Antiproliferative activity against human JAR cells assessed as reduction in cell viability at 10 uM by MTT assay | Homo sapiens | 8 | ALA5154666 | cell-based format | Scientific Literature |
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