# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5130697 | Binding | Inhibition of recombinant HDAC4 (unknown origin) using Boc-Lys(TFA)-AMC as substrate by fluorescence based plate reader assay | Homo sapiens | 10 | ALA5126633 | single protein format | Scientific Literature | |
2. | ALA5130698 | Binding | Inhibition of recombinant HDAC5 (unknown origin) using Boc-Lys(TFA)-AMC as substrate by fluorescence based plate reader assay | Homo sapiens | 10 | ALA5126633 | single protein format | Scientific Literature | |
3. | ALA5130699 | Binding | Inhibition of recombinant HDAC7 (unknown origin) using Boc-Lys(TFA)-AMC as substrate by fluorescence based plate reader assay | Homo sapiens | 10 | ALA5126633 | single protein format | Scientific Literature | |
4. | ALA5130700 | Binding | Inhibition of recombinant HDAC9 (unknown origin) using Boc-Lys(TFA)-AMC as substrate by fluorescence based plate reader assay | Homo sapiens | 10 | ALA5126633 | single protein format | Scientific Literature | |
5. | ALA5130701 | Binding | Inhibition of class 2a HDAC in human Jurkat E6.1 cells using Boc-Lys(TFA)-AMC as substrate incubated for 24 hrs by plate reader assay | Homo sapiens | 10 | ALA5126633 | cell-based format | Scientific Literature | |
6. | ALA5130702 | Binding | Inhibition of class 1 HDAC in human Jurkat E6.1 cells using Boc-Lys(Ac)-AMC as substrate incubated for 24 hrs by plate reader assay | Homo sapiens | 10 | ALA5126633 | cell-based format | Scientific Literature | |
7. | ALA5130703 | Physicochemical | Kinetic aqueous solubility of the compound in PBS buffer at pH 7.4 incubated for 2 hrs under shaking condition by LC-UV analysis | 10 | ALA5126633 | small-molecule physicochemical format | Scientific Literature | ||
8. | ALA5130704 | Binding | Protac activity at VHL/HDAC4 in human Jurkat E6.1 cells assessed as induction of HDAC4 degradation incubated for 24 hrs by ELISA-based MSD electrochemiluminescence assay | Homo sapiens | 8 | ALA5126633 | cell-based format | Scientific Literature | |
9. | ALA5130705 | Binding | Protac activity at VHL/HDAC4 in human Jurkat E6.1 cells assessed as induction of HDAC4 degradation by measuring maximum degradation at 10 uM incubated for 24 hrs by ELISA-based MSD electrochemiluminescence assay relative to control | Homo sapiens | 8 | ALA5126633 | cell-based format | Scientific Literature | |
10. | ALA5130707 | Binding | Protac activity at VHL/HDAC4 in human Jurkat E6.1 cells assessed as induction of HDAC4 degradation incubated for 24 hrs by immunoblotting analysis | Homo sapiens | 2 | ALA5126633 | cell-based format | Scientific Literature | |
11. | ALA5130710 | Binding | Protac activity at VHL/HDAC4 in human Jurkat E6.1 cells assessed as induction of HDAC4 degradation at 100 nM incubated for 6 hrs in presence of proteasome inhibitor MG132 by immunoblotting analysis | Homo sapiens | 1 | ALA5126633 | cell-based format | Scientific Literature | |
12. | ALA5130711 | Binding | Protac activity at VHL/HDAC4 in human Jurkat E6.1 cells assessed as induction of HDAC4 degradation at 100 nM incubated for 6 hrs in presence of proteasome inhibitor carfilzomib by immunoblotting analysis | Homo sapiens | 1 | ALA5126633 | cell-based format | Scientific Literature | |
13. | ALA5130712 | Binding | Protac activity at VHL/HDAC1 in human Jurkat E6.1 cells assessed as reduction in immunoreactivity at 0.05 to 10000 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5126633 | cell-based format | Scientific Literature | |
14. | ALA5130713 | Binding | Protac activity at VHL/HDAC3 in human Jurkat E6.1 cells assessed as reduction in immunoreactivity at 0.05 to 10000 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5126633 | cell-based format | Scientific Literature | |
15. | ALA5130714 | Binding | Protac activity at VHL/HDAC3 in human Jurkat E6.1 cells assessed as induction of HDAC3 degradation at 10 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5126633 | cell-based format | Scientific Literature | |
16. | ALA5130715 | Binding | Protac activity at VHL/HDAC5 in human Jurkat E6.1 cells assessed as reduction in immunoreactivity at 0.05 to 10000 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5126633 | cell-based format | Scientific Literature | |
17. | ALA5130716 | Binding | Protac activity at VHL/HDAC7 in human Jurkat E6.1 cells assessed as reduction in immunoreactivity at 0.05 to 10000 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5126633 | cell-based format | Scientific Literature | |
18. | ALA5130717 | Binding | Protac activity at VHL/HDAC9 in human Jurkat E6.1 cells assessed as reduction in immunoreactivity at 0.05 to 10000 nM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | ALA5126633 | cell-based format | Scientific Literature | |
19. | ALA5130721 | Binding | Protac activity at VHL/HDAC4 in human Jurkat E6.1 cells assessed as induction of HDAC4 degradation incubated for 24 hrs in absence of elacridar by Western blot analysis | Homo sapiens | 1 | ALA5126633 | cell-based format | Scientific Literature | |
20. | ALA5130722 | Binding | Protac activity at VHL/HDAC4 in human Jurkat E6.1 cells assessed as induction of HDAC4 degradation preincubated with elacridar for 1 hr followed by compound addition and measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA5126633 | cell-based format | Scientific Literature |