Assay

#	Aladdin ID	Assay Type	Description	Organism	Compounds	BAO Format	Source
1.	ALA5251097	B	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	22	single protein format	Scientific Literature
2.	ALA5251098	B	Inhibition of human USP21 using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	22	single protein format	Scientific Literature
3.	ALA5251115	P	Lipophilicity, logD of compound at pH 7.5 by HPCL analysis		22	small-molecule physicochemical format	Scientific Literature
4.	ALA5251116	P	Aqueous solubility of compound in PBS at pH 6.5 incubated for 24 hrs by HPLC-MS/MS analysis		12	small-molecule physicochemical format	Scientific Literature
5.	ALA5251117	B	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate assessed as maximal response preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	11	single protein format	Scientific Literature
6.	ALA5251118	B	Inhibition of human USP21 using ubiquitin rhodamine 110 as substrate assessed as maximal response preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	8	single protein format	Scientific Literature
7.	ALA5251121	A	Apparent permeability across apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6	cell-based format	Scientific Literature
8.	ALA5251122	A	Efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6	cell-based format	Scientific Literature