| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5253737 | B | Inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) at 100 uM by fluorescence polarization assay relative to control | Homo sapiens | 22 | single protein format | Scientific Literature | ||
| 2. | ALA5253738 | B | Inhibition of m7GDP fluorescent probe binding to recombinant eIF4E (unknown origin) assessed as dissociation constant by fluorescence polarization assay | Homo sapiens | 23 | single protein format | Scientific Literature | ||
| 3. | ALA5253739 | B | Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation at 100 uM preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis relative to control | Homo sapiens | 22 | single protein format | Scientific Literature | ||
| 4. | ALA5253740 | B | Inhibition of P32-[alpha-GTP] cap radiolabelled RNA binding to recombinant eIF4E (unknown origin) assessed as decrease in cross linked RNA-eIF4E complex formation preincubated with UV radiation for 30 mins followed by RNase addition and measured after 30 mins by SDS-PAGE based autoradiographic analysis | Homo sapiens | 23 | single protein format | Scientific Literature | ||
| 5. | ALA5253741 | B | Inhibition of eIF4E-mediated translation in luciferase RNA primed rabbit reticulocyte lysate assessed as reduction in luciferase production incubated for 90 mins by agarose gel electrophoresis | Oryctolagus cuniculus | 23 | subcellular format | Scientific Literature | ||
| 6. | ALA5253744 | F | Inhibition of protein synthesis in methionine-starved human HeLa cells incubated for 24 hrs by scintillation counting based 35S-Met incorporation assay | Homo sapiens | 23 | cell-based format | Scientific Literature | ||
| 7. | ALA5253745 | F | Inhibition of protein synthesis in methionine-starved human MCF7 cells incubated for 24 hrs by scintillation counting based 35S-Met incorporation assay | Homo sapiens | 23 | cell-based format | Scientific Literature |
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