# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5242984 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 54 | ALA5241107 | cell-based format | Scientific Literature | |
2. | ALA5242985 | B | Uncompetitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using varying concentrations of ATP and 250 ug/ml yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
3. | ALA5242986 | B | Uncompetitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as decrease in Km and Vmax of ATP using varying concentrations of ATP and 250 ug/ml yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
4. | ALA5242987 | B | Competitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using 250 uM ATP and varying concentration of yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
5. | ALA5242988 | B | Competitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as decrease in Km with constant Vmax of RNA using 250 uM ATP and varying concentration of yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
6. | ALA5242996 | F | Cytotoxicity against human BJAB cells assessed as reduction in cell viability measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 7 | ALA5241107 | cell-based format | Scientific Literature | |
7. | ALA5243007 | B | Displacement of FAM-A-[CAA]5 from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells preincubated with enzyme for 20 mins followed by FAM-A[CAA]5 addition for 30 mins by fluorescence polarization assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
8. | ALA5243010 | B | Inhibition of helicase activity of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as increase in double stranded signal using Cy3-labeled RNA strand and unlabeled DNA strand by native PAGE analysis | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
9. | ALA5243011 | B | Inhibition of helicase activity of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as decrease in single stranded signal using Cy3-labeled RNA strand and unlabeled DNA strand by native PAGE analysis | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
10. | ALA5243012 | B | Inhibition of helicase activity of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using Cy3-labeled RNA strand and unlabeled DNA strand by native PAGE analysis | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature |