# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5233078 | B | Inhibition of human recombinant tissue transglutaminase expressed in Escherichia coli using Cbz-Glu-(gamma-p-nitrophenylester)Gly (AL5) as substrate assessed as substrate hydrolysis by measuring Kinact incubated for 20 mins by colorimetric analysis | Homo sapiens | 12 | assay format | Scientific Literature | ||
2. | ALA5233079 | A | Metabolic stability in pooled cryopreserved human hepatocytes assessed as half-life at 1 uM measured upto 120 mins by LC-MS/MS analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
3. | ALA5233080 | A | Metabolic stability in human whole blood assessed as half-life at 1 to 5 uM measured upto 120 mins by LC-MS/MS analysis | Homo sapiens | 3 | tissue-based format | Scientific Literature | ||
4. | ALA5233084 | B | Irreversible inhibition of human recombinant tissue transglutaminase expressed in Escherichia coli using Cbz-Glu-(gamma-p-nitrophenylester)Gly (AL5) as substrate assessed as substrate hydrolysis by measuring KI incubated for 20 mins by colorimetric analysis | Homo sapiens | 12 | assay format | Scientific Literature | ||
5. | ALA5233088 | B | Irreversible inhibition of human recombinant tissue transglutaminase expressed in Escherichia coli using Cbz-Glu-(gamma-p-nitrophenylester)Gly (AL5) as substrate assessed as substrate hydrolysis by measuring Kinact/Ki ratio incubated for 20 mins by colorimetric analysis | Homo sapiens | 11 | assay format | Scientific Literature | ||
6. | ALA5233090 | B | Irreversible inhibition of human recombinant tissue transglutaminase expressed in Escherichia coli using Cbz-Glu-(gamma-p-nitrophenylester)Gly (AL5) as substrate assessed as substrate hydrolysis by measuring Kinact/Ki ratio by linear regression analysis | Homo sapiens | 11 | assay format | Scientific Literature | ||
7. | ALA5233092 | P | GSH reactivity in aqueous buffer assessed as GSH adduct formation by measuring corrected intrinsic second order rate constant (K2 corrected) by HPLC analysis | 3 | small-molecule physicochemical format | Scientific Literature | |||
8. | ALA5233093 | B | Irreversible inhibition of human TG2 using Cbz-Glu-(gamma-p-nitrophenylester)Gly (AL5) as substrate assessed as substrate hydrolysis by measuring observed binding constant incubated for 20 mins by colorimetric assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
9. | ALA5233094 | B | Irreversible inhibition of human TG1 using Cbz-Glu-(gamma-p-nitrophenylester)Gly (AL5) as substrate assessed as substrate hydrolysis by measuring observed binding constant incubated for 100 mins by colorimetric assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
10. | ALA5233095 | B | Irreversible inhibition of human TG6 using Cbz-Glu-(gamma-p-nitrophenylester)Gly (AL5) as substrate assessed as substrate hydrolysis by measuring observed binding constant incubated for 60 mins by colorimetric assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
11. | ALA5233096 | B | Reversible inhibition of activated human TG3 using peptidic FRET-quenched probe A101 as substrate by fluorescence based assay relative to control | Homo sapiens | 4 | single protein format | Scientific Literature | ||
12. | ALA5233098 | B | Reversible inhibition of human factor XIIIa using peptidic FRET-quenched probe A101 as substrate by fluorescence based assay relative to control | Homo sapiens | 1 | single protein format | Scientific Literature | ||
13. | ALA5233101 | P | Ratio of Kinact/Ki for inhibition of human tissue transglutaminase assessed as substrate hydrolysis to corrected second order rate constant (K2 corrected) for GSH reactivity in aqueous buffer assessed as GSH adduct formation | 3 | small-molecule physicochemical format | Scientific Literature |